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240302s2024 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c02941
|2 doi
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|a (NLM)38427698
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|a DE-627
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|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
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|a Sahu, Anand Kumar
|e verfasserin
|4 aut
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|a Exploring the Physical Properties of Lipid Membranes with Polyhydroxy Oxanorbornane Head Group Using NBD-Conjugated and DPH Fluorescent Probes
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.03.2024
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|a Date Revised 08.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The present study focuses on exploring the physical properties of lipid membranes based on the polyhydroxy oxanorbornane (PH-ONB) headgroup, designed as synthetic analogues of naturally occurring archaeal lipid membranes. Specifically, we study two variants of PH-ONB headgroup-based lipids differing in the number of hydroxy groups present in the headgroup, with one having two hydroxy groups (ONB-2OH) and the other having three (ONB-3OH). These lipids form stable bilayer membranes. The study begins with a comprehensive analysis of the fluorescence characteristics of nitrobenzoxadiazole (NBD)-tagged ONB-based lipids in different solvent environments and within a model lipid membrane 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). Subsequently, the physical properties of the ONB-based membranes were examined by using an NBD-tagged ONB-based probe and a commonly used extrinsic 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescent probe. The steady-state and time-resolved fluorescence properties of the NBD-tagged ONB-based probe and DPH were used to compare the physical properties of the ONB-based membranes, including polarity, fluidity, phase transition, order, hydration, location, heterogeneity, and rotational diffusion. The solid gel to liquid crystalline phase transition temperatures of ONB-2OH and ONB-3OH lipid membranes are found to be (68 ± 1) °C and (74 ± 1) °C, respectively. The variation in organization (size), fluidity, and phase transition temperature of ONB-based lipid membranes is explained by the extent of hydrogen bonding interactions between lipid head groups. ONB-based membranes exhibit characteristics similar to those of phospholipid membranes and possess a notably high phase transition temperature. These properties make them a promising and cost-effective synthetic alternative to archaeal lipid membranes with a wide range of potential applications
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fluorescent Dyes
|2 NLM
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|a Phospholipids
|2 NLM
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|a Lipid Bilayers
|2 NLM
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| 650 |
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|a Phosphatidylcholines
|2 NLM
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| 700 |
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|a Reddy, U Chandrasekhar
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kannoth Manheri, Muraleedharan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mishra, Ashok Kumar
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 40(2024), 10 vom: 12. März, Seite 5106-5120
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
1 |
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|g volume:40
|g year:2024
|g number:10
|g day:12
|g month:03
|g pages:5106-5120
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|u http://dx.doi.org/10.1021/acs.langmuir.3c02941
|3 Volltext
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