Acid phosphatase involved in phosphate homeostasis in Brassica napus and the functional analysis of BnaPAP10s

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 208(2024) vom: 26. März, Seite 108389
1. Verfasser:	Zhang, Hao (VerfasserIn)
Weitere Verfasser:	He, Xuyou, Munyaneza, Venuste, Zhang, Guangzeng, Ye, Xiangsheng, Wang, Chuang, Shi, Lei, Wang, Xu, Ding, Guangda
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article BnaPAP10as Brassica napus Expression profile Phosphate homeostasis Purple acid phosphatases Root-associated APase activity Acid Phosphatase EC 3.1.3.2 Phosphates Plant Proteins

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier Masson SAS. All rights reserved. Purple acid phosphatases (PAPs) are involved in activating the rhizosphere's organic phosphorus (P) and promoting P recycling during plant development, especially under the long-term P deficiency conditions in acid soil. However, the function of BnaPAPs in response to P deficiency stress in Brassica napus has rarely been explored. In this study, we found that the acid phosphatase activities (APA) of rapeseed shoot and root increased under P deficienct conditions. Genome-wide identification found that 82 PAP genes were unevenly distributed on 19 chromosomes in B. napus, which could be divided into eight subfamilies. The segmental duplication events were the main driving force for expansion during evolution, and the gene structures and conserved motifs of most members within the same subfamily were highly conservative. Moreover, the expression levels of 37 and 23 different expressed genes were induced by low P in leaf and root, respectively. BnaA09.PAP10a and BnaC09.PAP10a were identified as candidate genes via interaction networks. Significantly, both BnaPAP10a overexpression lines significantly increased root-related APA and total phosphate concentration under P deficiency and ATP supply conditions, thereby improving plant growth and root length. In summary, our results provided a valuable foundation for further study of BnaPAP functions
Beschreibung:	Date Completed 01.04.2024 Date Revised 01.04.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2024.108389