Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia

Bibliographische Detailangaben
Veröffentlicht in:	Clinical immunology (Orlando, Fla.). - 1999. - 261(2024) vom: 22. Apr., Seite 109942
1. Verfasser:	Abraham, Roshini S (VerfasserIn)
Weitere Verfasser:	Basu, Amrita, Heimall, Jennifer R, Dunn, Elizabeth, Yip, Alison, Kapadia, Malika, Kapoor, Neena, Satter, Lisa Forbes, Buckley, Rebecca, O'Reilly, Richard, Cuvelier, Geoffrey D E, Chandra, Sharat, Bednarski, Jeffrey, Chaudhury, Sonali, Moore, Theodore B, Haines, Hilary, Dávila Saldaña, Blachy J, Chellapandian, Deepakbabu, Rayes, Ahmad, Chen, Karin, Caywood, Emi, Chandrakasan, Shanmuganathan, Lugt, Mark Thomas Vander, Ebens, Christen, Teira, Pierre, Shereck, Evan, Miller, Holly, Aquino, Victor, Eissa, Hesham, Yu, Lolie C, Gillio, Alfred, Madden, Lisa, Knutsen, Alan, Shah, Ami J, DeSantes, Kenneth, Barnum, Jessie, Broglie, Larisa, Joshi, Avni Y, Kleiner, Gary, Dara, Jasmeen, Prockop, Susan, Martinez, Caridad, Mousallem, Talal, Oved, Joseph, Burroughs, Lauri, Marsh, Rebecca, Torgerson, Troy R, Leiding, Jennifer W, Pai, Sung Yun, Kohn, Donald B, Pulsipher, Michael A, Griffith, Linda M, Notarangelo, Luigi D, Cowan, Morton J, Puck, Jennifer, Dvorak, Christopher C, Haddad, Elie
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Clinical immunology (Orlando, Fla.)
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Mitogen PHA PIDTC SCID Severe combined immunodeficiency T-cell lymphopenia T-cell proliferation

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier Inc. All rights reserved. Severe combined immunodeficiency (SCID) is characterized by a severe deficiency in T cell numbers. We analyzed data collected (n = 307) for PHA-based T cell proliferation from the PIDTC SCID protocol 6901, using either a radioactive or flow cytometry method. In comparing the two groups, a smaller number of the patients tested by flow cytometry had <10% of the lower limit of normal proliferation as compared to the radioactive method (p = 0.02). Further, in patients with CD3+ T cell counts between 51 and 300 cells/μL, there was a higher proliferative response with the PHA flow assay compared to the 3H-T assay (p < 0.0001), suggesting that the method of analysis influences the resolution and interpretation of PHA results. Importantly, we observed many SCID patients with profound T cell lymphopenia having normal T cell proliferation when assessed by flow cytometry. We recommend this test be considered only as supportive in the diagnosis of typical SCID
Beschreibung:	Date Completed 18.03.2024 Date Revised 25.04.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-7035
DOI:	10.1016/j.clim.2024.109942