Suberin deficiency and its effect on the transport physiology of young poplar roots

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 242(2024), 1 vom: 17. März, Seite 137-153
1. Verfasser:	Grünhofer, Paul (VerfasserIn)
Weitere Verfasser:	Heimerich, Ines, Pohl, Svenja, Oertel, Marlene, Meng, Hongjun, Zi, Lin, Lucignano, Kevin, Bokhari, Syed Nadeem Hussain, Guo, Yayu, Li, Ruili, Lin, Jinxing, Fladung, Matthias, Kreszies, Tino, Stöcker, Tyll, Schoof, Heiko, Schreiber, Lukas
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article CRISPR Casparian bands apoplastic barriers hydraulic conductivity poplar adventitious root suberin lamellae transcriptomics water and nutrient transport suberin mehr... 8072-95-5 Lipids Water 059QF0KO0R

Beschreibung
Zusammenfassung:	© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation. The precise functions of suberized apoplastic barriers in root water and nutrient transport physiology have not fully been elucidated. While lots of research has been performed with mutants of Arabidopsis, little to no data are available for mutants of agricultural crop or tree species. By employing a combined set of physiological, histochemical, analytical, and transport physiological methods as well as RNA-sequencing, this study investigated the implications of remarkable CRISPR/Cas9-induced suberization defects in young roots of the economically important gray poplar. While barely affecting overall plant development, contrary to literature-based expectations significant root suberin reductions of up to 80-95% in four independent mutants were shown to not evidently affect the root hydraulic conductivity during non-stress conditions. In addition, subliminal iron deficiency symptoms and increased translocation of a photosynthesis inhibitor as well as NaCl highlight the involvement of suberin in nutrient transport physiology. The multifaceted nature of the root hydraulic conductivity does not allow drawing simplified conclusions such as that the suberin amount must always be correlated with the water transport properties of roots. However, the decreased masking of plasma membrane surface area could facilitate the uptake but also leakage of beneficial and harmful solutes
Beschreibung:	Date Completed 08.03.2024 Date Revised 08.03.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.19588