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240217s2024 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erae039
|2 doi
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|a pubmed24n1499.xml
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|a (NLM)38364822
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a Arce, Rocío C
|e verfasserin
|4 aut
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|a Introduction of a terminal electron sink in chloroplasts decreases leaf cell expansion associated with higher proteasome activity and lower endoreduplication
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 12.08.2024
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|a Date Revised 12.08.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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|a Foliar development involves successive phases of cell proliferation and expansion that determine the final leaf size, and is characterized by an early burst of reactive oxygen species generated in the photosynthetic electron transport chain (PETC). Introduction of the alternative PETC acceptor flavodoxin in tobacco chloroplasts led to a reduction in leaf size associated to lower cell expansion, without affecting cell number per leaf. Proteomic analysis showed that the biogenesis of the PETC proceeded stepwise in wild-type leaves, with accumulation of light-harvesting proteins preceding that of electron transport components, which might explain the increased energy and electron transfer to oxygen and reactive oxygen species build-up at this stage. Flavodoxin expression did not affect biogenesis of the PETC but prevented hydroperoxide formation through its function as electron sink. Mature leaves from flavodoxin-expressing plants were shown to contain higher levels of transcripts encoding components of the proteasome, a key negative modulator of organ size. Proteome profiling revealed that this differential accumulation was initiated during expansion and led to increased proteasomal activity, whereas a proteasome inhibitor reverted the flavodoxin-dependent size phenotype. Cells expressing plastid-targeted flavodoxin displayed lower endoreduplication, also associated to decreased organ size. These results provide novel insights into the regulation of leaf growth by chloroplast-generated redox signals, and highlight the potential of alternative electron shuttles to investigate the link(s) between photosynthesis and plant development
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|a Journal Article
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|a Electron transport
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|a endoreduplication
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|a flavodoxin
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|a leaf cell expansion
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|a leaf development
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|a leaf size
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|a photosynthesis
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|a proteasome
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|a proteomics
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|a reactive oxygen species
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|a Proteasome Endopeptidase Complex
|2 NLM
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| 650 |
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|a EC 3.4.25.1
|2 NLM
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| 650 |
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|a Flavodoxin
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Mayta, Martín L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Melzer, Michael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hajirezaei, Mohammad-Reza
|e verfasserin
|4 aut
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| 700 |
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|a Lodeyro, Anabella F
|e verfasserin
|4 aut
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| 700 |
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|a Carrillo, Néstor
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 15 vom: 12. Aug., Seite 4625-4640
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:75
|g year:2024
|g number:15
|g day:12
|g month:08
|g pages:4625-4640
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|u http://dx.doi.org/10.1093/jxb/erae039
|3 Volltext
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