The ETI-dependent receptor-like kinase 1 positively regulates effector-triggered immunity by stabilizing NLR-required for cell death 4 in Nicotiana benthamiana

© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 242(2024), 2 vom: 01. Apr., Seite 576-591
1. Verfasser: Sun, Yujing (VerfasserIn)
Weitere Verfasser: Liu, Fan, Zeng, Mengzhu, Zhang, Xinjie, Cui, Ying, Chen, Zhaodan, Wang, Lei, Xu, Yuanpeng, Wu, Jinbin, Guo, Shengya, Dong, Xian, Dong, Suomeng, Wang, Yan, Wang, Yuanchao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article ETI LRR‐RLK NRC4 immune signal transduction kinase activity plant immunity Leucine GMW67QNF9C
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520 |a Leucine-rich repeat receptor-like kinases (LRR-RLKs) comprise the largest class of membrane-localized receptor-like kinases in plants. Leucine-rich repeat receptor-like kinases are key immune sectors contributing to pattern-triggered immunity (PTI), but whether LRR-RLK mediates effector-triggered immunity (ETI) in plants remains unclear. In this study, we evaluated the function of LRR-RLKs in regulating ETI by using a virus-induced gene silencing (VIGS)-based reverse genetic screening assay, and identified a LRR-RLK named ETI-dependent receptor-like kinase 1 (EDK1) required for ETI triggered by the avirulence effector AVRblb2 secreted by Phytophthora infestans and its cognate receptor Rpi-blb2. Silencing or knockout of EDK1 compromised immunity mediated by Rpi-blb2 and the cell death triggered by recognition of AVRblb2. NLR-required for cell death 4 (NRC4), a signaling component acts downstream of Rpi-blb2, was identified that interacts with EDK1 using the LC-MS analysis and the interaction was further evaluated by co-immunoprecipitation. EDK1 promotes protein accumulation of NRC4 in a kinase-dependent manner and positively regulates resistance to P. infestans in Nicotiana benthamiana. Our study revealed that EDK1 positively regulates plant ETI through modulating accumulation of the NLR signaling component NRC4, representing a new regulatory role of the membrane-localized LRR-RLKs in plant immunity 
650 4 |a Journal Article 
650 4 |a ETI 
650 4 |a LRR‐RLK 
650 4 |a NRC4 
650 4 |a immune signal transduction 
650 4 |a kinase activity 
650 4 |a plant immunity 
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650 7 |a GMW67QNF9C  |2 NLM 
700 1 |a Liu, Fan  |e verfasserin  |4 aut 
700 1 |a Zeng, Mengzhu  |e verfasserin  |4 aut 
700 1 |a Zhang, Xinjie  |e verfasserin  |4 aut 
700 1 |a Cui, Ying  |e verfasserin  |4 aut 
700 1 |a Chen, Zhaodan  |e verfasserin  |4 aut 
700 1 |a Wang, Lei  |e verfasserin  |4 aut 
700 1 |a Xu, Yuanpeng  |e verfasserin  |4 aut 
700 1 |a Wu, Jinbin  |e verfasserin  |4 aut 
700 1 |a Guo, Shengya  |e verfasserin  |4 aut 
700 1 |a Dong, Xian  |e verfasserin  |4 aut 
700 1 |a Dong, Suomeng  |e verfasserin  |4 aut 
700 1 |a Wang, Yan  |e verfasserin  |4 aut 
700 1 |a Wang, Yuanchao  |e verfasserin  |4 aut 
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773 1 8 |g volume:242  |g year:2024  |g number:2  |g day:01  |g month:04  |g pages:576-591 
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