Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses

Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses

© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 242(2024), 1 vom: 13. März, Seite 170-191
1. Verfasser: Kumakura, Naoyoshi (VerfasserIn)
Weitere Verfasser: Singkaravanit-Ogawa, Suthitar, Gan, Pamela, Tsushima, Ayako, Ishihama, Nobuaki, Watanabe, Shunsuke, Seo, Mitsunori, Iwasaki, Shintaro, Narusaka, Mari, Narusaka, Yoshihiro, Takano, Yoshitaka, Shirasu, Ken
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Colletotrichum orbiculare effector pathogen-associated molecular pattern (PAMP) pattern-triggered immunity (PTI) ribonuclease Ribonucleases EC 3.1.-
Beschreibung
Zusammenfassung:© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.
Plants activate immunity upon recognition of pathogen-associated molecular patterns. Although phytopathogens have evolved a set of effector proteins to counteract plant immunity, some effectors are perceived by hosts and induce immune responses. Here, we show that two secreted ribonuclease effectors, SRN1 and SRN2, encoded in a phytopathogenic fungus, Colletotrichum orbiculare, induce cell death in a signal peptide- and catalytic residue-dependent manner, when transiently expressed in Nicotiana benthamiana. The pervasive presence of SRN genes across Colletotrichum species suggested the conserved roles. Using a transient gene expression system in cucumber (Cucumis sativus), an original host of C. orbiculare, we show that SRN1 and SRN2 potentiate host pattern-triggered immunity responses. Consistent with this, C. orbiculare SRN1 and SRN2 deletion mutants exhibited increased virulence on the host. In vitro analysis revealed that SRN1 specifically cleaves single-stranded RNAs at guanosine, leaving a 3'-end phosphate. Importantly, the potentiation of C. sativus responses by SRN1 and SRN2, present in the apoplast, depends on ribonuclease catalytic residues. We propose that the pathogen-derived apoplastic guanosine-specific single-stranded endoribonucleases lead to immunity potentiation in plants
Beschreibung:Date Completed 08.03.2024
Date Revised 08.03.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19582