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240213s2024 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-09-23-1827-RE
|2 doi
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|a pubmed25n1227.xml
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|a (DE-627)NLM36835685X
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|a (NLM)38345539
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Tambong, James T
|e verfasserin
|4 aut
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| 245 |
1 |
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|a TaqMan Real-Time PCR Assay for Specific Detection and Differentiation of Xanthomonas translucens pv. undulosa from Other Pathovars Targeting a Recombination Mediator Gene, recF
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| 264 |
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|c 2024
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 21.06.2024
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|a Date Revised 10.07.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a Bacterial leaf streak and black chaff diseases of wheat caused by Xanthomonas translucens pv. undulosa is becoming a major constraint to growers and trade since it is seedborne. Molecular tools for specific detection/differentiation of pv. undulosa are lacking. We report the development of a TaqMan real-time PCR for specific detection/identification of pv. undulosa targeting the recombination mediator gene (recF). Analysis of the complete recF (1,117 bp) sequences identified the gene as a reliable phylogenetic marker for identification of pv. undulosa, differentiating it from the other pathovars; recF-based sequence homology values among the 11 pathovars correlated well with genome-based DNA-DNA hybridization values. The discriminatory power of recF to differentiate pv. undulosa from the other pathovars is due to nucleotide polymorphic positions. We used these nucleotide polymorphisms to develop a TaqMan PCR for specific detection of pv. undulosa. The specificity of the assay was validated using 67 bacterial and fungal/oomycete strains. The selected primers and the double-quenched FAM-labeled TaqMan probe were specific for the detection of 11 pv. undulosa/secalis strains. The 56 strains of other X. translucens pathovars (n = 39) and non-Xanthomonas spp. (n = 17) did not exhibit any detectable fluorescence. Also, greenhouse-inoculated and naturally infected wheat leaf samples showed positive reactions for the presence of pv. undulosa DNA but not healthy control plants. The TaqMan assay reliably detected as low as 1-pg DNA amount and 10 colony forming units of the target pathogen per reaction. This TaqMan assay could be useful to regulatory agencies with economic benefits to wheat growers
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4 |
|a Journal Article
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| 650 |
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4 |
|a DNA-DNA hybridization
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| 650 |
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4 |
|a TaqMan assay
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| 650 |
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4 |
|a Xanthomonas translucens
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| 650 |
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4 |
|a barley
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| 650 |
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4 |
|a pathovars
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| 650 |
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4 |
|a pv. translucens
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| 650 |
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4 |
|a pv. undulosa
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| 650 |
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4 |
|a recF
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| 650 |
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4 |
|a wheat
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| 650 |
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4 |
|a whole-genome sequence
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| 650 |
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7 |
|a Bacterial Proteins
|2 NLM
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| 650 |
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7 |
|a DNA, Bacterial
|2 NLM
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| 700 |
1 |
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|a Xu, Renlin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fleitas, Maria Constanza
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Lipu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Akuma, Mercy
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chi, Sylvia I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kutcher, Hadley R
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 108(2024), 6 vom: 13. Juni, Seite 1869-1878
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnas
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| 773 |
1 |
8 |
|g volume:108
|g year:2024
|g number:6
|g day:13
|g month:06
|g pages:1869-1878
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-09-23-1827-RE
|3 Volltext
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|d 108
|j 2024
|e 6
|b 13
|c 06
|h 1869-1878
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