Dynamic N6 -methyladenosine RNA modification regulates peanut resistance to bacterial wilt

Dynamic N6 -methyladenosine RNA modification regulates peanut resistance to bacterial wilt

© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 242(2024), 1 vom: 20. März, Seite 231-246
1. Verfasser: Zhao, Kai (VerfasserIn)
Weitere Verfasser: Li, Zhongfeng, Ke, Yunzhuo, Ren, Rui, Cao, Zenghui, Li, Zhan, Wang, Kuopeng, Wang, Xiaoxuan, Wang, Jinzhi, Ma, Qian, Cao, Di, Zhao, Kunkun, Li, Yaoyao, Hu, Sasa, Qiu, Ding, Gong, Fangping, Ma, Xingli, Zhang, Xingguo, Fan, Guoqiang, Liang, Zhe, Yin, Dongmei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article bacterial wilt demethylase disease resistance m6A modification peanut RNA 63231-63-0
Beschreibung
Zusammenfassung:© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.
N6 -methyladenosine (m6 A) is the most abundant mRNA modification in eukaryotes and is an important regulator of gene expression as well as many other critical biological processes. However, the characteristics and functions of m6 A in peanut (Arachis hypogea L.) resistance to bacterial wilt (BW) remain unknown. Here, we analyzed the dynamic of m6 A during infection of resistant (H108) and susceptible (H107) peanut accessions with Ralstonia solanacearum (R. solanacearum), the causative agent of BW. Throughout the transcriptome, we identified 'URUAY' as a highly conserved motif for m6 A in peanut. The majority of differential m6 A located within the 3' untranslated region (UTR) of the transcript, with fewer in the exons. Integrative analysis of RNA-Seq and m6 A methylomes suggests the correlation between m6 A and gene expression in peanut R. solanacearum infection, and functional analysis reveals that m6 A-associated genes were related to plant-pathogen interaction. Our experimental analysis suggests that AhALKBH15 is an m6 A demethylase in peanut, leading to decreased m6 A levels and upregulation of the resistance gene AhCQ2G6Y. The upregulation of AhCQ2G6Y expression appears to promote BW resistance in the H108 accession
Beschreibung:Date Completed 08.03.2024
Date Revised 08.03.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19568