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240208s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2024.112015
|2 doi
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|a pubmed25n1226.xml
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|a (DE-627)NLM368148963
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|a (NLM)38325662
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|a (PII)S0168-9452(24)00042-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Wang, Xin
|e verfasserin
|4 aut
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|a PbWRKY18 promotes resistance against black spot disease by activation of the chalcone synthase gene PbCHS3 in pear
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 26.02.2024
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|a Date Revised 26.02.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier B.V. All rights reserved.
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|a Flavonoids are plant pigments that play a major role in plant defense and have significant health benefits to humans. Chalcone synthase (CHS) is an important enzyme in flavonoid biosynthesis and investigation transcription factors (TFs) regulating its expression and downstream targets is critical to understanding its mechanism. Here, a novel TF, PbWRKY18, was isolated from the pear Pyrus betulaefolia. Its expression was evaluated in various tissues by RT-PCR, particularly in response to Alternaria alternata, the pathogen responsible for black spot disease, and exogenous hormone administration. The PbWRKY18 protein was primarily found in the nucleus where it regulated transcriptional activity. Yeast one-hybrid and dual-luciferase reporter assays showed a strong association between PbWRKY18 and the PbCHS3 promoter, which drives PbCHS3 expression. It was also found that PbCHS3 was critical for the development of resistance against black spot disease. In addition, PbWRKY18 was found to significantly increase the expression of PbCHS3 and salicylic acid-related genes, as well as defense enzyme activity and tolerance to black spot disease. PbWRKY18 or PbCHS3 knockdown in pear attenuates resistance to Alternaria alternata. In summary, the study identified a novel WRKY18-CHS3 axis involved in resistance against black spot disease in pear
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|a Journal Article
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|a Alternaria alternata
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|a Black spot disease
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|a PbCHS3
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|a PbWRKY18
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|a Transcriptional activation
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|a flavanone synthetase
|2 NLM
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| 650 |
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|a EC 2.3.1.74
|2 NLM
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| 650 |
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|a Acyltransferases
|2 NLM
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| 650 |
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|a EC 2.3.-
|2 NLM
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| 700 |
1 |
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|a Qiao, Qinghai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Keke
|e verfasserin
|4 aut
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| 700 |
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|a Zhai, Wenhui
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dong, Huizhen
|e verfasserin
|4 aut
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| 700 |
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|a Lin, Likun
|e verfasserin
|4 aut
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1 |
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|a Xing, Caihua
|e verfasserin
|4 aut
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| 700 |
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|a Su, Zhiyuan
|e verfasserin
|4 aut
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| 700 |
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|a Pan, Zhijian
|e verfasserin
|4 aut
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|a Zhang, Shaoling
|e verfasserin
|4 aut
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| 700 |
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|a Huang, Xiaosan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 341(2024) vom: 10. Apr., Seite 112015
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
1 |
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|g volume:341
|g year:2024
|g day:10
|g month:04
|g pages:112015
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| 856 |
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|u http://dx.doi.org/10.1016/j.plantsci.2024.112015
|3 Volltext
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|d 341
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