Using XAS to monitor radiation damage in real time and post-analysis, and investigation of systematic errors of fluorescence XAS for Cu-bound amyloid-β

© Ruwini S. K. Ekanayake et al. 2024.

Bibliographische Detailangaben
Veröffentlicht in:Journal of applied crystallography. - 1998. - 57(2024), Pt 1 vom: 01. Feb., Seite 125-139
1. Verfasser: Ekanayake, Ruwini S K (VerfasserIn)
Weitere Verfasser: Streltsov, Victor A, Best, Stephen P, Chantler, Christopher T
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Journal of applied crystallography
Schlagworte:Journal Article EXAFS N-truncated amyloid pepides X-ray absorption spectro-electrochemical measurements XAS-EC dead-time correction extended X-ray absorption fine structure radiation damage
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245 1 0 |a Using XAS to monitor radiation damage in real time and post-analysis, and investigation of systematic errors of fluorescence XAS for Cu-bound amyloid-β 
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520 |a X-ray absorption spectroscopy (XAS) is a promising technique for determining structural information from sensitive biological samples, but high-accuracy X-ray absorption fine structure (XAFS) requires corrections of systematic errors in experimental data. Low-temperature XAS and room-temperature X-ray absorption spectro-electrochemical (XAS-EC) measurements of N-truncated amyloid-β samples were collected and corrected for systematic effects such as dead time, detector efficiencies, monochromator glitches, self-absorption, radiation damage and noise at higher wavenumber (k). A new protocol was developed using extended X-ray absorption fine structure (EXAFS) data analysis for monitoring radiation damage in real time and post-analysis. The reliability of the structural determinations and consistency were validated using the XAS measurement experimental uncertainty. The correction of detector pixel efficiencies improved the fitting χ2 by 12%. An improvement of about 2.5% of the structural fitting was obtained after dead-time corrections. Normalization allowed the elimination of 90% of the monochromator glitches. The remaining glitches were manually removed. The dispersion of spectra due to self-absorption was corrected. Standard errors of experimental measurements were propagated from pointwise variance of the spectra after systematic corrections. Calculated uncertainties were used in structural refinements for obtaining precise and reliable values of structural parameters including atomic bond lengths and thermal parameters. This has permitted hypothesis testing 
650 4 |a Journal Article 
650 4 |a EXAFS 
650 4 |a N-truncated amyloid pepides 
650 4 |a X-ray absorption spectro-electrochemical measurements 
650 4 |a XAS-EC 
650 4 |a dead-time correction 
650 4 |a extended X-ray absorption fine structure 
650 4 |a radiation damage 
700 1 |a Streltsov, Victor A  |e verfasserin  |4 aut 
700 1 |a Best, Stephen P  |e verfasserin  |4 aut 
700 1 |a Chantler, Christopher T  |e verfasserin  |4 aut 
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