|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM368056104 |
| 003 |
DE-627 |
| 005 |
20250305184252.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
240206s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plantsci.2024.112009
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1226.xml
|
| 035 |
|
|
|a (DE-627)NLM368056104
|
| 035 |
|
|
|a (NLM)38316345
|
| 035 |
|
|
|a (PII)S0168-9452(24)00036-0
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Qiao, Qinghai
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a PuNDH9, a subunit of ETC Complex I regulates plant defense by interacting with PuPR1
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.02.2024
|
| 500 |
|
|
|a Date Revised 26.02.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2024 Elsevier B.V. All rights reserved.
|
| 520 |
|
|
|a NAD+ and NADH play critical roles in energy metabolism, cell death, and gene expression. The NADH-ubiquinone oxidoreductase complex (Complex I) has been long known as a key enzyme in NAD+ and NADH metabolism. In the present study, we found and analyzed a new subunit of Complex I (NDH9), which was isolated from Pyrus ussuriensis combined with RT-PCR. Following infection with A. alternata, RT-qPCR analysis demonstrated an increase in the expression of PuNDH9. Genetic manipulation of PuNDH9 levels suggested that PuNDH9 plays key roles in NADH/NAD+ homeostasis, defense enzyme activities, ROS generation, cell death, gene expression, energy metabolism, and mitochondrial functions during the pear- A. alternata interaction. Furthermore, Y2H, GST-pull down, and a split-luciferase complementation imaging assays revealed that PuNDH9 interacts with PuPR1. We discover that PuNDH9 and PuPR1 synergistically activate defense enzyme activities, ROS accumulation, cell death, and plant defenses. Collectively, our findings reveal that PuNDH9 is likely important for plant defenses
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Complex I
|
| 650 |
|
4 |
|a Plant defense
|
| 650 |
|
4 |
|a PuNDH9
|
| 650 |
|
4 |
|a PuPR1
|
| 650 |
|
4 |
|a ROS
|
| 650 |
|
7 |
|a Electron Transport Complex I
|2 NLM
|
| 650 |
|
7 |
|a EC 7.1.1.2
|2 NLM
|
| 650 |
|
7 |
|a NAD
|2 NLM
|
| 650 |
|
7 |
|a 0U46U6E8UK
|2 NLM
|
| 650 |
|
7 |
|a Reactive Oxygen Species
|2 NLM
|
| 700 |
1 |
|
|a Wang, Xin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Su, Zhiyuan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Han, Chenyang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhao, Keke
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Qi, Kaijie
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xie, Zhihua
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Huang, Xiaosan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Shaoling
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 341(2024) vom: 15. Apr., Seite 112009
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
|
| 773 |
1 |
8 |
|g volume:341
|g year:2024
|g day:15
|g month:04
|g pages:112009
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plantsci.2024.112009
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 341
|j 2024
|b 15
|c 04
|h 112009
|