Active oxygen generation induced by the glucose sensor TaHXK7-1A decreased the drought resistance of transgenic Arabidopsis and wheat (Triticum aestivum L.)

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 207(2024) vom: 01. Feb., Seite 108410
1. Verfasser: Tang, Peng (VerfasserIn)
Weitere Verfasser: Shan, Sicong, Wang, Rui, Xu, Wei, Yan, Nuo, Niu, Na, Zhang, Gaisheng, Gao, Xin, Min, Donghong, Song, Yulong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Drought stress ROS TaHXK7-1A Wheat Reactive Oxygen Species Luciferases EC 1.13.12.- Glucose IY9XDZ35W2 Plant Proteins
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520 |a Improving wheat drought resistance is of great significance for grain production and food security. Hexokinases (HXKs) play a role in sugar signal transduction and are involved in abiotic stress responses in wheat. To clarify the relationship between HXKs and drought stress in wheat, we used the rice active oxygen induction gene OsHXK1 as a reference sequence and the homologously cloned wheat TaHXK7-1A gene. TaHXK7-1A was localized in the nucleus and cell membrane. Under drought stress, over-expression of TaHXK7-1A increased the contents of O2·- and malondialdehyde (MDA) and significantly up-regulated the respiratory burst oxidative homologue (RBOHs) genes in transgenic Arabidopsis. In addition, the over-expression of TaHXK7-1A inhibited the growth of Arabidopsis seedlings and increased ROS accumulation under 6 % exogenous glucose treatment. Gene silencing of TaHXK7-1 decreased the contents of O2·- and MDA in wheat leaves under drought stress, and the RBOHs was significantly down-regulated, which improved the drought resistance of wheat. The results of yeast one-hybrid, EMSA, and dual-luciferase assays showed that TabHLH148-5A bound to the E-box motif of the TaHXK7-1A promoter and inhibited the expression of TaHXK7-1A. In addition, yeast two-hybrid and luciferase complementation imaging assays showed that TaHXK7-1A interacted with TaGRF3-4A. These results indicate that the glucose sensor TaHXK7-1A was negatively regulated by TabHLH148-5A, interacted with TaGRF3-4A, and negatively regulated wheat drought resistance by regulating RBOHs expression and inducing ROS production, thus providing a theoretical basis for revealing the molecular mechanism of wheat drought resistance 
650 4 |a Journal Article 
650 4 |a Drought stress 
650 4 |a ROS 
650 4 |a TaHXK7-1A 
650 4 |a Wheat 
650 7 |a Reactive Oxygen Species  |2 NLM 
650 7 |a Luciferases  |2 NLM 
650 7 |a EC 1.13.12.-  |2 NLM 
650 7 |a Glucose  |2 NLM 
650 7 |a IY9XDZ35W2  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Shan, Sicong  |e verfasserin  |4 aut 
700 1 |a Wang, Rui  |e verfasserin  |4 aut 
700 1 |a Xu, Wei  |e verfasserin  |4 aut 
700 1 |a Yan, Nuo  |e verfasserin  |4 aut 
700 1 |a Niu, Na  |e verfasserin  |4 aut 
700 1 |a Zhang, Gaisheng  |e verfasserin  |4 aut 
700 1 |a Gao, Xin  |e verfasserin  |4 aut 
700 1 |a Min, Donghong  |e verfasserin  |4 aut 
700 1 |a Song, Yulong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 207(2024) vom: 01. Feb., Seite 108410  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnas 
773 1 8 |g volume:207  |g year:2024  |g day:01  |g month:02  |g pages:108410 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2024.108410  |3 Volltext 
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