CRISPR/Cas9-mediated homology donor repair base editing system to confer herbicide resistance in maize (Zea mays L.)

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 207(2024) vom: 01. Feb., Seite 108374
1. Verfasser: Kaul, Tanushri (VerfasserIn)
Weitere Verfasser: Thangaraj, Arulprakash, Jain, Rashmi, Bharti, Jyotsna, Kaul, Rashmi, Verma, Rachana, Sony, Sonia Khan, Abdel Motelb, Khaled Fathy, Yadav, Pranjal, Agrawal, Pawan Kumar
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Aromatic amino acid EPSPS Glyphosate Homology donor repair Maize 4632WW1X5A Herbicides Amino Acids, Aromatic
LEADER 01000caa a22002652 4500
001 NLM368002225
003 DE-627
005 20240318234213.0
007 cr uuu---uuuuu
008 240205s2024 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.plaphy.2024.108374  |2 doi 
028 5 2 |a pubmed24n1334.xml 
035 |a (DE-627)NLM368002225 
035 |a (NLM)38310724 
035 |a (PII)S0981-9428(24)00042-1 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Kaul, Tanushri  |e verfasserin  |4 aut 
245 1 0 |a CRISPR/Cas9-mediated homology donor repair base editing system to confer herbicide resistance in maize (Zea mays L.) 
264 1 |c 2024 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 18.03.2024 
500 |a Date Revised 18.03.2024 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2024 Elsevier Masson SAS. All rights reserved. 
520 |a Weed infestation is a significant concern to crop yield loss, globally. The potent broad-spectrum glyphosate (N-phosphomethyl-glycine) has a widely utilized herbicide, acting on the shikimic acid pathway within chloroplast by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). This crucial enzyme plays a vital role in aromatic amino acid synthesis. Repurposing of CRISPR/Cas9-mediated gene-editing was the inflection point for generating novel crop germplasm with diverse genetic variations in essential agronomic traits, achieved through the introduction of nucleotide substitutions at target sites within the native genes, and subsequent induction of indels through error-prone non-homologous end-joining DNA repair mechanisms. Here, we describe the development of efficient herbicide-resistant maize lines by using CRISPR/Cas9 mediated site-specific native ZmEPSPS gene fragment replacement via knock-out of conserved region followed by knock-in of desired homologous donor repair (HDR-GATIPS-mZmEPSPS) with triple amino acid substitution. The novel triple substitution conferred high herbicide tolerance in edited maize plants. Transgene-free progeny harbouring the triple amino acid substitutions revealed agronomic performances similar to that of wild-type plants, suggesting that the GATIPS-mZmEPSPS allele substitutions are crucial for developing elite maize varieties with significantly enhanced glyphosate resistance. Furthermore, the aromatic amino acid contents in edited maize lines were significantly higher than in wild-type plants. The present study describing the introduction of site-specific CRISPR/Cas9- GATIPS mutations in the ZmEPSPS gene via genome editing has immense potential for higher tolerance to glyphosate with no yield penalty in maize 
650 4 |a Journal Article 
650 4 |a Aromatic amino acid 
650 4 |a EPSPS 
650 4 |a Glyphosate 
650 4 |a Homology donor repair 
650 4 |a Maize 
650 7 |a Glyphosate  |2 NLM 
650 7 |a 4632WW1X5A  |2 NLM 
650 7 |a Herbicides  |2 NLM 
650 7 |a Amino Acids, Aromatic  |2 NLM 
700 1 |a Thangaraj, Arulprakash  |e verfasserin  |4 aut 
700 1 |a Jain, Rashmi  |e verfasserin  |4 aut 
700 1 |a Bharti, Jyotsna  |e verfasserin  |4 aut 
700 1 |a Kaul, Rashmi  |e verfasserin  |4 aut 
700 1 |a Verma, Rachana  |e verfasserin  |4 aut 
700 1 |a Sony, Sonia Khan  |e verfasserin  |4 aut 
700 1 |a Abdel Motelb, Khaled Fathy  |e verfasserin  |4 aut 
700 1 |a Yadav, Pranjal  |e verfasserin  |4 aut 
700 1 |a Agrawal, Pawan Kumar  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 207(2024) vom: 01. Feb., Seite 108374  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:207  |g year:2024  |g day:01  |g month:02  |g pages:108374 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2024.108374  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 207  |j 2024  |b 01  |c 02  |h 108374