Epigenetic immune cell quantification for diagnostic evaluation and monitoring of patients with inborn errors of immunity and secondary immune deficiencies

Epigenetic immune cell quantification for diagnostic evaluation and monitoring of patients with inborn errors of immunity and secondary immune deficiencies

Copyright © 2023. Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 260(2024) vom: 01. März, Seite 109920
1. Verfasser: Ramirez, Neftali J (VerfasserIn)
Weitere Verfasser: Schulze, Janika J, Walter, Steffi, Werner, Jeannette, Mrovecova, Pavla, Olek, Sven, Sachsenmaier, Christoph, Grimbacher, Bodo, Salzer, Ulrich
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Epigenetic quantification Flow cytometry Inborn errors of immunity Primary immunodeficiencies Secondary immunodeficiencies Edetic Acid 9G34HU7RV0
Beschreibung
Zusammenfassung:Copyright © 2023. Published by Elsevier Inc.
BACKGROUND: Early detection and monitoring of primary immunodeficiencies (PID) in humans require quantitative determination of immune cells from fresh blood analyzed by flow cytometry. However, epigenetic immune cell quantification allows analysis from fresh, frozen, or dried blood samples. We demonstrate the utility of epigenetic immune cell quantification for patients with PID
METHODS: Epigenetic quantification of basic lymphocyte subpopulations of 259 samples from PID patients were compared to flow cytometric data. Epigenetic analysis was extended to T-cell subsets (Treg, Th17, Tfh, PD-1+, CCR6+) and memory B-cells and compared between venous EDTA and dried blood
RESULTS: A high correlation of >0.9 was observed for basic T- and B-cell subsets. Extended epigenetic analysis showed quantitative trends within PID subgroups, but individually these varied substantially within these groups. Epigenetic analysis of dried blood samples was equivalent to EDTA blood
CONCLUSION: Epigenetic immune cell quantification is suitable for immune cell profiling in PID patients
Beschreibung:Date Completed 19.02.2024
Date Revised 10.04.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2024.109920