High-throughput screening for a SARS-CoV-2 frameshifting inhibitor using a cell-free protein synthesis system
Programmed-1 ribosomal frameshifting (-1 PRF) is a translational mechanism adopted by some viruses, including SARS-CoV-2. To find a compound that can inhibit -1 PRF in SARS-CoV-2, we set up a high-throughput screening system using a HeLa cell extract-derived cell-free protein synthesis (CFPS) system...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 76(2024), 4 vom: 25. März, Seite 161-168
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| 1. Verfasser: |
Machida, Kodai
(VerfasserIn) |
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| Weitere Verfasser: |
Tanaka, Rin,
Miki, Seraya,
Noseda, Shotaro,
Yuasa-Sunagawa, Mayumi,
Imataka, Hiroaki |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
SARS-CoV-2
cell-free protein synthesis
drug discovery
programmed-1 ribosomal frameshifting
screening |
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