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240131s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.108395
|2 doi
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|a pubmed24n1334.xml
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|a (DE-627)NLM367806614
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|a (PII)S0981-9428(24)00063-9
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|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Ye, Xu
|e verfasserin
|4 aut
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|a CsSPL13A directly binds and positively regulates CsFT and CsBAM to accelerate flowering in cucumber
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 18.03.2024
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|a Date Revised 18.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
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|a Flowering is an important developmental transition that greatly affects the yield of many vegetable crops. In cucumber (Cucumis sativus), flowering is regulated by various factors including squamosa promoter-binding-like (SPL) family proteins. However, the role of CsSPL genes in cucumber flowering remains largely unknown. In this study, we cloned the squamosa promoter-binding-like protein 13A (CsSPL13A) gene, which encodes a highly conserved SBP-domain protein that acts as a transcription factor and localizes to the nucleus. Quantitative real-time PCR (qRT-PCR) analysis showed that CsSPL13A was mainly expressed in flowers, and its expression level increased significantly nearing the flowering stage. Additionally, compared with the wild type(WT), CsSPL13A-overexpressing transgenic cucumber plants (CsSPL13A-OE) showed considerable differences in flowering phenotypes, such as early flowering, increased number of male flowers, and longer flower stalks. CsSPL13A upregulated the expression of the flowering integrator gene Flowering Locus T (CsFT) and the sugar-mediated flowering gene β-amylase (CsBAM) in cucumber. Yeast one-hybrid and firefly enzyme reporter assays confirmed that the CsSPL13A protein could directly bind to the promoters of CsFT and CsBAM, suggesting that CsSPL13A works together with CsFT and CsBAM to mediate flowering in cucumber. Overall, our results provide novel insights into the regulatory network of flowering in cucumber as well as new ideas for the genetic improvement of cucumber varieties
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|a Journal Article
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|a CsBAM
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|a CsFT
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|a CsSPL
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|a Cucumber
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|a flowering
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|a Transcription Factors
|2 NLM
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|a Deng, Qinlin
|e verfasserin
|4 aut
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|a Xu, Shicheng
|e verfasserin
|4 aut
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|a Huang, Yifang
|e verfasserin
|4 aut
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|a Wei, Dayong
|e verfasserin
|4 aut
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|a Wang, Zhimin
|e verfasserin
|4 aut
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|a Zhang, Hongcheng
|e verfasserin
|4 aut
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|a Wang, Hebing
|e verfasserin
|4 aut
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|a Tang, Qinglin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 207(2024) vom: 26. Feb., Seite 108395
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:207
|g year:2024
|g day:26
|g month:02
|g pages:108395
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|u http://dx.doi.org/10.1016/j.plaphy.2024.108395
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