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|a 10.1002/adma.202309026
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|a eng
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|a Riffe, Morgan B
|e verfasserin
|4 aut
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|a Multi-Material Volumetric Additive Manufacturing of Hydrogels using Gelatin as a Sacrificial Network and 3D Suspension Bath
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|c 2024
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|a Text
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|a ƒaComputermedien
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|a Date Revised 23.08.2024
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a © 2024 Wiley‐VCH GmbH.
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|a Volumetric additive manufacturing (VAM) is an emerging layerless method for the rapid processing of reactive resins into 3D structures, where printing is much faster (seconds) than other lithography and direct ink writing methods (minutes to hours). As a vial of resin rotates in the VAM process, patterned light exposure defines a 3D object and then resin that has not undergone gelation can be washed away. Despite the promise of VAM, there are challenges with the printing of soft hydrogel materials from non-viscous precursors, including multi-material constructs. To address this, sacrificial gelatin is used to modulate resin viscosity to support the cytocompatible VAM printing of macromers based on poly(ethylene glycol) (PEG), hyaluronic acid (HA), and polyacrylamide (PA). After printing, gelatin is removed by washing at an elevated temperature. To print multi-material constructs, the gelatin-containing resin is used as a shear-yielding suspension bath (including HA to further modulate bath properties) where ink can be extruded into the bath to define a multi-material resin that can then be processed with VAM into a defined object. Multi-material constructs of methacrylated HA (MeHA) and gelatin methacrylamide (GelMA) are printed (as proof-of-concept) with encapsulated mesenchymal stromal cells (MSCs), where the local hydrogel properties guide cell spreading behavior with culture
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|a Journal Article
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|a hydrogels
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|a suspension bath printing
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|a tissue engineering
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| 650 |
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|a volumetric additive manufacturing
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1 |
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|a Davidson, Matthew D
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1 |
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|a Seymour, Gabriel
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1 |
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|a Dhand, Abhishek P
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1 |
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|a Cooke, Megan E
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|a Zlotnick, Hannah M
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1 |
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|a McLeod, Robert R
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1 |
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|a Burdick, Jason A
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|t Advanced materials (Deerfield Beach, Fla.)
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