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240108s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.108302
|2 doi
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|a pubmed24n1293.xml
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|a (DE-627)NLM366617524
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|a (NLM)38171134
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|a DE-627
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|e rakwb
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|a eng
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|a Cheng, Hongtao
|e verfasserin
|4 aut
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|a Targeted mutagenesis of BnTTG1 homologues generated yellow-seeded rapeseed with increased oil content and seed germination under abiotic stress
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.02.2024
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|a Date Revised 14.02.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier Masson SAS. All rights reserved.
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|a Yellow seed is one desirable trait with great potential to improve seed oil quality and yield. The present study surveys the redundant role of BnTTG1 genes in the proanthocyanidins (PA) biosynthesis, oil content and abiotic stress resistance. Stable yellow seed mutants were generated after mutating BnTTG1 by CRISPR/Cas9 genome editing system. Yellow seed phenotype could be obtained only when both functional homologues of BnTTG1 were simultaneously knocked out. Homozygous mutants of BnTTG1 homologues showed decreased thickness and PA accumulation in seed coat. Transcriptome and qRT-PCR analysis indicated that BnTTG1 mutation inhibited the expression of genes involved in phenylpropanoid and flavonoid biosynthetic pathways. Increased seed oil content and alteration of fatty acid (FA) composition were observed in homozygous mutants of BnTTG1 with enriched expression of genes involved in FA biosynthesis pathway. In addition, target mutation of BnTTG1 accelerated seed germination rate under salt and cold stresses. Enhanced seed germination capacity in BnTTG1 mutants was correlated with the change of expression level of ABA responsive genes. Overall, this study elucidated the redundant role of BnTTG1 in regulating seed coat color and established an efficient approach for generating yellow-seeded oilseed rape genetic resources with increase oil content, modified FA composition and resistance to multiple abiotic stresses
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|a Journal Article
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|a BnTTG1
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|a Flavonoids
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|a Gene editing
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|a Oil content
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|a Seed germination
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|a Yellow seed
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| 650 |
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|a Plant Oils
|2 NLM
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| 700 |
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|a Cai, Shengli
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hao, Mengyu
|e verfasserin
|4 aut
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| 700 |
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|a Cai, Yating
|e verfasserin
|4 aut
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| 700 |
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|a Wen, Yunfei
|e verfasserin
|4 aut
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| 700 |
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|a Huang, Wei
|e verfasserin
|4 aut
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| 700 |
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|a Mei, Desheng
|e verfasserin
|4 aut
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| 700 |
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|a Hu, Qiong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 206(2024) vom: 14. Jan., Seite 108302
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:206
|g year:2024
|g day:14
|g month:01
|g pages:108302
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|u http://dx.doi.org/10.1016/j.plaphy.2023.108302
|3 Volltext
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