Intrinsically disordered Prosystemin discloses biologically active repeat motifs

Intrinsically disordered Prosystemin discloses biologically active repeat motifs

Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 340(2024) vom: 22. Feb., Seite 111969
1. Verfasser: Castaldi, Valeria (VerfasserIn)
Weitere Verfasser: Langella, Emma, Buonanno, Martina, Di Lelio, Ilaria, Aprile, Anna Maria, Molisso, Donata, Criscuolo, Martina Chiara, D'Andrea, Luca Domenico, Romanelli, Alessandra, Amoresano, Angela, Pinto, Gabriella, Illiano, Anna, Chiaiese, Pasquale, Becchimanzi, Andrea, Pennacchio, Francesco, Rao, Rosa, Monti, Simona Maria
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Defense Disorder Gene expression In vivo detection Mimetics Plant Repeats Peptides Plant Proteins
Beschreibung
Zusammenfassung:Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
The in-depth studies over the years on the defence barriers by tomato plants have shown that the Systemin peptide controls the response to a wealth of environmental stress agents. This multifaceted stress reaction seems to be related to the intrinsic disorder of its precursor protein, Prosystemin (ProSys). Since latest findings show that ProSys has biological functions besides Systemin sequence, here we wanted to assess if this precursor includes peptide motifs able to trigger stress-related pathways. Candidate peptides were identified in silico and synthesized to test their capacity to trigger defence responses in tomato plants against different biotic stressors. Our results demonstrated that ProSys harbours several repeat motifs which triggered plant immune reactions against pathogens and pest insects. Three of these peptides were detected by mass spectrometry in plants expressing ProSys, demonstrating their effective presence in vivo. These experimental data shed light on unrecognized functions of ProSys, mediated by multiple biologically active sequences which may partly account for the capacity of ProSys to induce defense responses to different stress agents
Beschreibung:Date Completed 05.02.2024
Date Revised 05.02.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2023.111969