Metatranscriptomics sheds light on the links between the functional traits of fungal guilds and ecological processes in forest soil ecosystems

Metatranscriptomics sheds light on the links between the functional traits of fungal guilds and ecological processes in forest soil ecosystems

© 2023 His Majesty the King in Right of Canada and The Authors. New Phytologist © 2023 New Phytologist Foundation Reproduced with the permission of the Minister of Natural Resources Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in th...

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Veröffentlicht in:The New phytologist. - 1979. - 242(2024), 4 vom: 01. Apr., Seite 1676-1690
1. Verfasser: Auer, Lucas (VerfasserIn)
Weitere Verfasser: Buée, Marc, Fauchery, Laure, Lombard, Vincent, Barry, Kerry W, Clum, Alicia, Copeland, Alex, Daum, Chris, Foster, Brian, LaButti, Kurt, Singan, Vasanth, Yoshinaga, Yuko, Martineau, Christine, Alfaro, Manuel, Castillo, Federico J, Imbert, J Bosco, Ramírez, Lucia, Castanera, Raúl, Pisabarro, Antonio G, Finlay, Roger, Lindahl, Björn, Olson, Ake, Séguin, Armand, Kohler, Annegret, Henrissat, Bernard, Grigoriev, Igor V, Martin, Francis M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't forest soil functional traits fungal guilds metatranscriptomics organic matter degradation Nitrogen N762921K75 Soil RNA, Messenger
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Zusammenfassung:© 2023 His Majesty the King in Right of Canada and The Authors. New Phytologist © 2023 New Phytologist Foundation Reproduced with the permission of the Minister of Natural Resources Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Soil fungi belonging to different functional guilds, such as saprotrophs, pathogens, and mycorrhizal symbionts, play key roles in forest ecosystems. To date, no study has compared the actual gene expression of these guilds in different forest soils. We used metatranscriptomics to study the competition for organic resources by these fungal groups in boreal, temperate, and Mediterranean forest soils. Using a dedicated mRNA annotation pipeline combined with the JGI MycoCosm database, we compared the transcripts of these three fungal guilds, targeting enzymes involved in C- and N mobilization from plant and microbial cell walls. Genes encoding enzymes involved in the degradation of plant cell walls were expressed at a higher level in saprotrophic fungi than in ectomycorrhizal and pathogenic fungi. However, ectomycorrhizal and saprotrophic fungi showed similarly high expression levels of genes encoding enzymes involved in fungal cell wall degradation. Transcripts for N-related transporters were more highly expressed in ectomycorrhizal fungi than in other groups. We showed that ectomycorrhizal and saprotrophic fungi compete for N in soil organic matter, suggesting that their interactions could decelerate C cycling. Metatranscriptomics provides a unique tool to test controversial ecological hypotheses and to better understand the underlying ecological processes involved in soil functioning and carbon stabilization
Beschreibung:Date Completed 25.04.2024
Date Revised 25.04.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19471