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231229s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2023.154160
|2 doi
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|a pubmed25n1220.xml
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|a (DE-627)NLM366384635
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|a (NLM)38147808
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|a (PII)S0176-1617(23)00254-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Li, Lili
|e verfasserin
|4 aut
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|a Genome-wide identification of the melon (Cucumis melo L.) response regulator gene family and functional analysis of CmRR6 and CmPRR3 in response to cold stress
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.02.2024
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|a Date Revised 14.02.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier GmbH. All rights reserved.
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|a The response regulator (RR) gene family play crucial roles in cytokinin signal transduction, plant development, and resistance to abiotic stress. However, there are no reports on the identification and functional characterization of RR genes in melon. In this study, a total of 18 CmRRs were identified and classified into type A, type B, and clock PRRs, based on phylogenetic analysis. Most of the CmRRs displayed tissue-specific expression patterns, and some were induced by cold stress according to two RNA-seq datasets. The expression patterns of CmRR2/6/11/15 and CmPRR2/3 under cold treatment were confirmed by qRT-PCR. Subcellular localization assays indicated that CmRR6 and CmPRR3 were primarily localized in the nucleus and chloroplast. Furthermore, when either CmRR6 or CmPRR3 were silenced using tobacco ringspot virus (TRSV), the cold tolerance of the virus-induced gene silencing (VIGS) melon plants were significantly enhanced, as evidenced by measurements of chlorophyll fluorescence, ion leakage, reactive oxygen, proline, and malondialdehyde levels. Additionally, the expression levels of CmCBF1, CmCBF2, and CmCBF3 were significantly increased in CmRR6-silenced and CmPRR3-silenced plants under cold treatment. Our findings suggest that CmRRs contribute to cold stress responses and provide new insights for further pursuing the molecular mechanisms underlying CmRRs-mediated cold tolerance in melon
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|a Journal Article
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|a Cold response
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|a Functional analysis
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|a Gene family
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|a Melon
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|a Response regulator
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|a Zhang, Xiuyue
|e verfasserin
|4 aut
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|a Ding, Fei
|e verfasserin
|4 aut
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|a Hou, Juan
|e verfasserin
|4 aut
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|a Wang, Jiyu
|e verfasserin
|4 aut
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|a Luo, Renren
|e verfasserin
|4 aut
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|a Mao, Wenwen
|e verfasserin
|4 aut
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|a Li, Xiang
|e verfasserin
|4 aut
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|a Zhu, Huayu
|e verfasserin
|4 aut
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|a Yang, Luming
|e verfasserin
|4 aut
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|a Li, Ying
|e verfasserin
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|a Hu, Jianbin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 292(2024) vom: 15. Jan., Seite 154160
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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| 773 |
1 |
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|g volume:292
|g year:2024
|g day:15
|g month:01
|g pages:154160
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|u http://dx.doi.org/10.1016/j.jplph.2023.154160
|3 Volltext
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