Molecular quantification of parasitic sea louse larvae depends on species and life stage

Molecular quantification of parasitic sea louse larvae depends on species and life stage

© 2023 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 47(2024), 4 vom: 26. Apr., Seite e13908
1. Verfasser: Mertz, Nathan E (VerfasserIn)
Weitere Verfasser: Paterson, Rachel A, Finstad, Bengt, Brandsegg, Hege, Andersskog, Ida Pernille Øystese, Fossøy, Frode
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article Caligus Lepeophtheirus copepodid ddPCR eDNA DNA 9007-49-2
Beschreibung
Zusammenfassung:© 2023 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd.
Sea lice cause substantial economic and environmental harm to Norway's aquaculture industry and wild salmonid populations. Rapid, accurate quantification of lice larval densities in coastal waters remains the greatest bottleneck for providing empirical data on infestation risk within wild salmon habitats and aquaculture production regions. We evaluated the capability of droplet digital PCR (ddPCR) as an absolute quantification method for the planktonic stages of two parasitic louse species, Lepeophtheirus salmonis (Krøyer) and Caligus elongatus (von Nordman). Results demonstrated linear relationships between the DNA quantity measured and the number of spiked larvae for both species and life stages. However, L. salmonis contained a significantly greater number of DNA copies than C. elongatus individuals and for C. elongatus, nauplii displayed a significantly higher number of DNA copies than copepodids. Our results suggest that ddPCR can effectively enumerate louse larvae, but interpreting ddPCR results differ between the two louse species. Obtaining larval abundance estimates from marine plankton samples will depend on the nauplii to copepodid ratio for C. elongatus, but not for L. salmonis
Beschreibung:Date Completed 11.03.2024
Date Revised 11.03.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1365-2761
DOI:10.1111/jfd.13908