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231227s2024 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2023-0087
|2 doi
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|a eng
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| 100 |
1 |
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|a Ma, Yingchun
|e verfasserin
|4 aut
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| 245 |
1 |
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|a An optimal genomic DNA extraction method for shoots of four Dendrocalamus species based on membership function analysis
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|c 2024
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a Date Completed 19.03.2024
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|a Date Revised 19.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a High-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that the commercial DNA extraction kit method was the most effective and comprehensive approach for extracting genomic DNA from shoots of four Dendrocalamus species. Furthermore, this study provided valuable insights into optimizing techniques for extracting genomic DNA in other bamboo species
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|a Journal Article
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|a Dendrocalamus
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|a bamboo
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|a extraction
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|a genomic DNA
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|a membership function method
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|a DNA
|2 NLM
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| 650 |
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|2 NLM
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| 700 |
1 |
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|a Li, Bowen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Dejia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Shuguang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Lixia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhan, Hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Juan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 76(2024), 3 vom: 08. März, Seite 94-103
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
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|g volume:76
|g year:2024
|g number:3
|g day:08
|g month:03
|g pages:94-103
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