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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.19461
|2 doi
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|a pubmed25n1218.xml
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|a (DE-627)NLM365748021
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|a (NLM)38084038
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Santin, Anna
|e verfasserin
|4 aut
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| 245 |
1 |
4 |
|a The tonoplast localized protein PtNPF1 participates in the regulation of nitrogen response in diatoms
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 26.01.2024
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|a Date Revised 06.02.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
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|a Diatoms are a highly successful group of phytoplankton, well adapted also to oligotrophic environments and capable of handling nutrient fluctuations in the ocean, particularly nitrate. The presence of a large vacuole is an important trait contributing to their adaptive features. It confers diatoms the ability to accumulate and store nutrients, such as nitrate, when they are abundant outside and then to reallocate them into the cytosol to meet deficiencies, in a process called luxury uptake. The molecular mechanisms that regulate these nitrate fluxes are still not known in diatoms. In this work, we provide new insights into the function of Phaeodactylum tricornutum NPF1, a putative low-affinity nitrate transporter. To accomplish this, we generated overexpressing strains and CRISPR/Cas9 loss-of-function mutants. Microscopy observations confirmed predictions that PtNPF1 is localized on the vacuole membrane. Furthermore, functional characterizations performed on knock-out mutants revealed a transient growth delay phenotype linked to altered nitrate uptake. Together, these results allowed us to hypothesize that PtNPF1 is presumably involved in modulating intracellular nitrogen fluxes, managing intracellular nutrient availability. This ability might allow diatoms to fine-tune the assimilation, storage and reallocation of nitrate, conferring them a strong advantage in oligotrophic environments
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4 |
|a Journal Article
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| 650 |
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4 |
|a Phaeodactylum tricornutum
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| 650 |
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4 |
|a diatom NPFs
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| 650 |
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4 |
|a genome editing
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| 650 |
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4 |
|a luxury uptake
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| 650 |
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|a nitrogen
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|a vacuole
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| 650 |
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|a Nitrates
|2 NLM
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| 650 |
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|a Nitrogen
|2 NLM
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| 650 |
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7 |
|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Russo, Monia Teresa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de Los Ríos, Laura Morales
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chiurazzi, Maurizio
|e verfasserin
|4 aut
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| 700 |
1 |
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|a d'Alcalà, Maurizio Ribera
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lacombe, Benoît
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ferrante, Maria Immacolata
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rogato, Alessandra
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 241(2024), 4 vom: 21. Feb., Seite 1592-1604
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
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|g volume:241
|g year:2024
|g number:4
|g day:21
|g month:02
|g pages:1592-1604
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|u http://dx.doi.org/10.1111/nph.19461
|3 Volltext
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|d 241
|j 2024
|e 4
|b 21
|c 02
|h 1592-1604
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