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20240214233002.0 |
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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.108255
|2 doi
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|a pubmed24n1293.xml
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|a (DE-627)NLM365626120
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|a (NLM)38071803
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|a (PII)S0981-9428(23)00766-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Amara, Umme
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a ECT12, an YTH-domain protein, is a potential mRNA m6A reader that affects abiotic stress responses by modulating mRNA stability in Arabidopsis
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|c 2024
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.02.2024
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|a Date Revised 14.02.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier Masson SAS. All rights reserved.
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|a N6-methyladenosine (m6A), the most abundant modification found in eukaryotic mRNAs, is interpreted by m6A "readers," thus playing a crucial role in regulating RNA metabolism. The YT521-B homology-domain (YTHD) proteins, also known as EVOLUTIONARILY CONSERVED C-TERMINAL REGION (ECT), are recognized as m6A reader proteins in plants and animals. Among the 13 potential YTHD family proteins in Arabidopsis thaliana, the functions of only a few members are known. In this study, we determined the function of ECT12 (YTH11) as a potential m6A reader that plays a crucial role in response to abiotic stresses. The loss-of-function ect12 mutants showed no noticeable developmental defects under normal conditions but displayed hypersensitivity to salt or dehydration stress. The salt- or dehydration-hypersensitive phenotypes were correlated with altered levels of several m6A-modified stress-responsive transcripts. Notably, the increased or decreased transcript levels were associated with each transcript's reduced or enhanced decay, respectively. Electrophoretic mobility shift and RNA-immunoprecipitation assays showed that ECT12 binds to m6A-modified RNAs both in vitro and in planta, suggesting its role as an m6A reader. Collectively, these results indicate that the potential m6A reader ECT12 regulates the stability of m6A-modified RNA transcripts, thereby facilitating the response of Arabidopsis to abiotic stresses
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|a Journal Article
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|a Abiotic stress
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|a Arabidopsis thaliana
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| 650 |
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|a ECT12
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|a RNA methylation
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| 650 |
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4 |
|a m(6)A reader
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| 650 |
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7 |
|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a RNA
|2 NLM
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| 650 |
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7 |
|a 63231-63-0
|2 NLM
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| 650 |
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|a Sodium Chloride
|2 NLM
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| 650 |
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7 |
|a 451W47IQ8X
|2 NLM
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| 700 |
1 |
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|a Hu, Jianzhong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Park, Su Jung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Hunseung
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 206(2024) vom: 08. Jan., Seite 108255
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
8 |
|g volume:206
|g year:2024
|g day:08
|g month:01
|g pages:108255
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2023.108255
|3 Volltext
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