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|a 10.1093/jxb/erad478
|2 doi
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|a pubmed24n1355.xml
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|a eng
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|a Im, Soo Hyun
|e verfasserin
|4 aut
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|a Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum
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|c 2024
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.03.2024
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|a Date Revised 29.03.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Aureochromes (AUREOs) are unique blue light receptors and transcription factors found only in stramenopile algae. While each of the four AUREOs identified in the diatom Phaeodactylum tricornutum may have a specific function, PtAUREO1a has been shown to have a strong impact on overall gene regulation, when light changes from red to blue light conditions. Despite its significance, the molecular mechanism of PtAUREO1a is largely unexplored. To comprehend the overall process of gene regulation by PtAUREO1a, we conducted a series of in vitro and in vivo experiments, including pull-down assays, yeast one-hybrid experiments, and phenotypical characterization using recombinant PtAUREOs and diatom mutant lines expressing a modified PtAureo1a gene. We describe the distinct light absorption properties of four PtAUREOs and the formation of all combinations of their potential dimers. We demonstrate the capability of PtAUREO1a and 1b to activate the genes, diatom-specific cyclin 2, PtAureo1a, and PtAureo1c under both light and dark conditions. Using mutant lines expressing a modified PtAUREO1a protein with a considerably reduced light absorption, we found novel evidence that PtAUREO1a regulates the expression of PtLHCF15, which is essential for red light acclimation. Based on current knowledge, we present a working model of PtAUREO1a gene regulation properties
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|a Journal Article
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|a Phaeodactylum tricornutum
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|a Aureochromes
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|a blue light receptors
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|a diatoms
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|a protein–protein interactions
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4 |
|a red-shifted antenna complex
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| 650 |
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4 |
|a yeast one-hybrid
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1 |
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|a Lepetit, Bernard
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mosesso, Niccolò
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shrestha, Sandeep
|e verfasserin
|4 aut
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| 700 |
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|a Weiss, Laura
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nymark, Marianne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Roellig, Robert
|e verfasserin
|4 aut
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| 700 |
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|a Wilhelm, Christian
|e verfasserin
|4 aut
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|a Isono, Erika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kroth, Peter G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 7 vom: 27. März, Seite 1834-1851
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:75
|g year:2024
|g number:7
|g day:27
|g month:03
|g pages:1834-1851
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|u http://dx.doi.org/10.1093/jxb/erad478
|3 Volltext
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