|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM365574503 |
| 003 |
DE-627 |
| 005 |
20250305131436.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erad490
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1218.xml
|
| 035 |
|
|
|a (DE-627)NLM365574503
|
| 035 |
|
|
|a (NLM)38066636
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Li, Zhi
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 28.03.2024
|
| 500 |
|
|
|a Date Revised 29.03.2024
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a NRT2.1, the major high affinity nitrate transporter in roots, can be phosphorylated at five different sites within the N- and the C-terminus. Here, we characterized the functional relationship of two N-terminal phosphorylation sites, S21 and S28, in Arabidopsis. Based on a site-specific correlation network, we identified a receptor kinase (HPCAL1, AT5G49770), phosphorylating NRT2.1 at S21 and resulting in active nitrate uptake. HPCAL1 itself was regulated by phosphorylation at S839 and S870 within its kinase domain. In the active state, when S839 was dephosphorylated and S870 was phosphorylated, HPCAL1 was found to interact with the N-terminus of NRT2.1, mainly when S28 was dephosphorylated. Phosphorylation of NRT2.1 at S21 resulted in a reduced interaction of NRT2.1 with its activator NAR2.1, but nitrate transport activity remained. By contrast, phosphorylated NRT2.1 at S28 enhanced the interaction with NAR2.1, but reduced the interaction with HPCAL1. Here we identified HPCAL1 as the kinase affecting this phospho-switch through phosphorylation of NRT2.1 at S21
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Kinase HPCAL1
|
| 650 |
|
4 |
|a nitrate
|
| 650 |
|
4 |
|a nitrate transporter NRT2.1
|
| 650 |
|
4 |
|a plasma membrane
|
| 650 |
|
4 |
|a post-translational regulation
|
| 650 |
|
4 |
|a protein phosphorylation
|
| 650 |
|
7 |
|a Nitrates
|2 NLM
|
| 650 |
|
7 |
|a Anion Transport Proteins
|2 NLM
|
| 650 |
|
7 |
|a Arabidopsis Proteins
|2 NLM
|
| 650 |
|
7 |
|a Nitrate Transporters
|2 NLM
|
| 700 |
1 |
|
|a Na Wu, Xu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jacquot, Aurore
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chaput, Valentin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Adamo, Mattia
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Neuhäuser, Benjamin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Straub, Tatsiana
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lejay, Laurence
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Schulze, Waltraud X
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 7 vom: 27. März, Seite 2127-2142
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
|
| 773 |
1 |
8 |
|g volume:75
|g year:2024
|g number:7
|g day:27
|g month:03
|g pages:2127-2142
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erad490
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 75
|j 2024
|e 7
|b 27
|c 03
|h 2127-2142
|