Polydopamine on Copper-Doped Cerium Dioxide Nanosheets as Peroxidase Mimics for the Intelligent Detection of Cholesterol

The catalytic ability of nanozymes has become an enzymology hotspot in the field of application. Most nanozymes were characterized to simultaneously have oxidase-like and peroxidase-like activities, but the practical application often focuses on certain activity; other complex activities may cause i...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 39(2023), 48 vom: 05. Dez., Seite 17286-17294
1. Verfasser: Mao, Yingmin (VerfasserIn)
Weitere Verfasser: Wang, Louqun, Zhang, Kai, Zhang, Hao, Yang, Jing
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Peroxidase EC 1.11.1.7 Copper 789U1901C5 ceric oxide 619G5K328Y polydopamine Hydrogen Peroxide mehr... BBX060AN9V Peroxidases EC 1.11.1.- Cerium 30K4522N6T Coloring Agents Cholesterol 97C5T2UQ7J
Beschreibung
Zusammenfassung:The catalytic ability of nanozymes has become an enzymology hotspot in the field of application. Most nanozymes were characterized to simultaneously have oxidase-like and peroxidase-like activities, but the practical application often focuses on certain activity; other complex activities may cause interference. The peroxidase-like activity (POD-like activity) of nanozymes have been widely used in the colorimetric detection of H2O2 or substances producing H2O2 as an intermediate, such as the detection of small biological molecules with the oxidative reaction of a chromogenic reagent in the presence of POD-like nanozymes. In this work, we used polydopamine (PDA) as the surface coating of Cu-CeO2 nanosheets (PDA Cu-CeO2), which enhanced peroxidase-like activity while inhibiting their oxidase-like activity, providing a feasible method for the sensitive determination of cholesterol by integrating visual colorimetric detection and a smartphone application as a readout. The absorbance intensity and RGB values displayed a linear range on cholesterol from 0.05 to 1.2 mM with the LOD (limit of detection) of 42.7 and 99.4 μM. In addition, the method is expected to apply in detecting cholesterol in human serum with acceptable accuracy
Beschreibung:Date Completed 06.12.2023
Date Revised 12.12.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/acs.langmuir.3c02372