Genome-wide identification of GROWTH-REGULATING FACTORs in Liriodendron chinense and functional characterization of LcGRF2 in leaf size regulation

Copyright © 2023 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 206(2024) vom: 02. Jan., Seite 108204
1. Verfasser: Wang, Jing (VerfasserIn)
Weitere Verfasser: Tu, Zhonghua, Wang, Minxin, Zhang, Yu, Hu, Qinghua, Li, Huogen
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Cell proliferation GRF transcription factors family Genetic transformation Leaf size Liriodendron chinense Arabidopsis Proteins
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520 |a GROWTH-REGULATING FACTORs (GRFs) play a pivotal role in the regulation of leaf size in plants and have been widely reported in plants. However, their specific functions in leaf size regulation in Liriodendron chinense remains unclear. Therefore, in this study, we identified GRF genes on a genome-wide scale in L. chinense to characterize the roles of LcGRFs in regulating leaf size. A total of nine LcGRF genes were identified, and these genes exhibited weak expression in mature leaves but strong expression in shoot apex. Notably, LcGRF2 exhibited the highest expression level in the shoot apex of L. chinense. Further RT-qPCR assay revealed that the expression level of LcGRF2 gradually decreased along with the leaf development process, and also displayed a gradient along the leaf proximo-distal and medio-lateral axes. Furthermore, overexpression of LcGRF2 in Arabidopsis thaliana resulted in increased leaf size, and significantly up-regulated the expression of genes involved in cell division like AtCYCD3;1, AtKNOLLE, and AtCYCB1;1, indicating that LcGRF2 may influence leaf size by promoting cell proliferation. This work contributes to a better understanding of the roles and molecular mechanisms of LcGRFs in the regulation of leaf size in L. chinense 
650 4 |a Journal Article 
650 4 |a Cell proliferation 
650 4 |a GRF transcription factors family 
650 4 |a Genetic transformation 
650 4 |a Leaf size 
650 4 |a Liriodendron chinense 
650 7 |a Arabidopsis Proteins  |2 NLM 
700 1 |a Tu, Zhonghua  |e verfasserin  |4 aut 
700 1 |a Wang, Minxin  |e verfasserin  |4 aut 
700 1 |a Zhang, Yu  |e verfasserin  |4 aut 
700 1 |a Hu, Qinghua  |e verfasserin  |4 aut 
700 1 |a Li, Huogen  |e verfasserin  |4 aut 
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