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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2023.111932
|2 doi
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|a pubmed25n1216.xml
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|a (DE-627)NLM365211796
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|a (NLM)38030037
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|a (PII)S0168-9452(23)00349-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Sougrakpam, Yaiphabi
|e verfasserin
|4 aut
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| 245 |
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|a Identification of nitric oxide regulated low abundant myrosinases from seeds and seedlings of Brassica juncea
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.01.2024
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|a Date Revised 15.01.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier B.V. All rights reserved.
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|a Myrosinases constitute an important component of the glucosinolate-myrosinase system responsible for interaction of plants with microorganisms, insects, pest, and herbivores. It is a distinctive feature of Brassicales. Multiple isozymes of myrosinases are present in the vacuoles. Active myrosinases are also present in the apoplast and the nucleus however, the similarity or difference in the biochemical properties with the vacuolar myrosinases are not known. Here, we have attempted to isolate, characterize, and identify myrosinases from seeds, seedlings, apoplast, and nucleus to understand these forms. 2D-CN/SDS-PAGE coupled with western blotting and MS have shown low abundant myrosinases (65/70/72/75 kDa) in seeds and seedlings and apoplast & nucleus of seedlings to exist as dimers, oligomers, and as protein complex. Nuclear membrane associated form of myrosinase was also identified. The present study for the first time has shown enzymatically active myrosinase-alpha-mannosidase complex in seedlings. Both 65 and 70 kDa myrosinase in seedlings were S-nitrosated. Nitric oxide donor treatment (GSNO) led to 25% reduction in myrosinase activity which was reversed by DTT suggesting redox regulation of myrosinase. These S-nitrosated myrosinases might be a component of NO signalling in B. juncea
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|a Journal Article
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|a 2D-CN/SDS-PAGE
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|a Apoplast
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|a Brassica juncea
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|a Myrosinase isoforms
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|a Nucleus
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|a S-nitrosation
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|a thioglucosidase
|2 NLM
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|a EC 3.2.1.147
|2 NLM
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|a Nitric Oxide
|2 NLM
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|a 31C4KY9ESH
|2 NLM
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|a Glycoside Hydrolases
|2 NLM
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|a EC 3.2.1.-
|2 NLM
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|a Glucosinolates
|2 NLM
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|a Deswal, Renu
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 339(2024) vom: 01. Feb., Seite 111932
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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|g volume:339
|g year:2024
|g day:01
|g month:02
|g pages:111932
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|u http://dx.doi.org/10.1016/j.plantsci.2023.111932
|3 Volltext
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