Automated method for quantification of 20 amino acids in cell culture media during biopharmaceutical development

Herein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn derivatization strategy using ortho-phthalaldehyde and 9-fluoren...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1993. - 76(2024), 1 vom: 06. Jan., Seite 27-36
1. Verfasser:	Dubey, Ankita (VerfasserIn)
Weitere Verfasser:	Joshi, Srishti, Upadhyay, Kratika, Mahato, Ansuman, Rathore, Anurag S
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't CHO media FMOC OPA amino acid analysis automation biopharmaceuticals liquid chromatography mammalian cell culture mehr... Amino Acids o-Phthalaldehyde 643-79-8 Amines Biological Products