Pseudomonas syringae coffee blight is associated with the horizontal transfer of plasmid-encoded type III effectors

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 241(2023), 1 vom: 11. Jan., Seite 409-429
1. Verfasser:	McTavish, Kathryn J (VerfasserIn)
Weitere Verfasser:	Almeida, Renan N D, Tersigni, Jonathan, Raimundi, Melina K, Gong, Yunchen, Wang, Pauline W, Gontijo, Guilherme F, de Souza, Ricardo M, de Resende, Mario L V, Desveaux, Darrell, Guttman, David S
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Pseudomonas syringae bacterial coffee blight disease comparative and evolutionary genomics genome-wide association study (GWAS) horizontal transfer host jump plasmid type III secreted effector Coffee

Beschreibung
Zusammenfassung:	© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. The emergence of new pathogens is an ongoing threat to human health and agriculture. While zoonotic spillovers received considerable attention, the emergence of crop diseases is less well studied. Here, we identify genomic factors associated with the emergence of Pseudomonas syringae bacterial blight of coffee. Fifty-three P. syringae strains from diseased Brazilian coffee plants were sequenced. Comparative and evolutionary analyses were used to identify loci associated with coffee blight. Growth and symptomology assays were performed to validate the findings. Coffee isolates clustered in three lineages, including primary phylogroups PG3 and PG4, and secondary phylogroup PG11. Genome-wide association study of the primary PG strains identified 37 loci, including five effectors, most of which were encoded on a plasmid unique to the PG3 and PG4 coffee strains. Evolutionary analyses support the emergence of coffee blight in PG4 when the coffee-associated plasmid and associated effectors derived from a divergent plasmid carried by strains associated with other hosts. This plasmid was only recently transferred into PG3. Natural diversity and CRISPR-Cas9 plasmid curing were used to show that strains with the coffee-associated plasmid grow to higher densities and cause more severe disease symptoms in coffee. This work identifies possible evolutionary mechanisms underlying the emergence of a new lineage of coffee pathogens
Beschreibung:	Date Completed 11.12.2023 Date Revised 11.12.2023 published: Print-Electronic RefSeq: AY603979.1, AY603980.1, AE016855.1, AE016854.1, MN346704.1, NZ_KY362367.1 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.19364