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|a 10.1093/jxb/erad443
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|a eng
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|a Hou, Xin
|e verfasserin
|4 aut
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|a Reducing PHYTOENE SYNTHASE activity fine-tunes the abundance of a cis-carotene-derived signal that regulates the PIF3/HY5 module and plastid biogenesis
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|c 2024
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 14.02.2024
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|a Date Revised 30.09.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a PHYTOENE SYNTHASE (PSY) is a rate-limiting enzyme catalysing the first committed step of carotenoid biosynthesis, and changes in PSY gene expression and/or protein activity alter carotenoid composition and plastid differentiation in plants. Four genetic variants of PSY (psy-4, psy-90, psy-130, and psy-145) were identified using a forward genetics approach that rescued leaf virescence phenotypes and plastid abnormalities displayed by the Arabidopsis CAROTENOID ISOMERASE (CRTISO) mutant ccr2 (carotenoid and chloroplast regulation 2) when grown under a shorter photoperiod. The four non-lethal mutations affected alternative splicing, enzyme-substrate interactions, and PSY:ORANGE multi-enzyme complex binding, constituting the dynamic post-transcriptional fine-tuning of PSY levels and activity without changing localization to the stroma and protothylakoid membranes. psy genetic variants did not alter total xanthophyll or β-carotene accumulation in ccr2, yet they reduced specific acyclic linear cis-carotenes linked to the biosynthesis of a currently unidentified apocarotenoid signal regulating plastid biogenesis, chlorophyll biosynthesis, and photomorphogenic regulation. ccr2 psy variants modulated the PHYTOCHROME-INTERACTING FACTOR 3/ELONGATED HYPOCOTYL 5 (PIF3/HY5) ratio, and displayed a normal prolamellar body formation in etioplasts and chlorophyll accumulation during seedling photomorphogenesis. Thus, suppressing PSY activity and impairing PSY:ORANGE protein interactions revealed how cis-carotene abundance can be fine-tuned through holoenzyme-metabolon interactions to control plastid development
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a cis-carotene
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|a Apocarotenoid signal
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|a Arabidopsis
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|a CAROTENOID ISOMERASE
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|a ORANGE
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|a PHYTOENE SYNTHASE
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|a photomorphogenesis
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|a plastid biogenesis
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|a skotomorphogenesis
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|a (all-E) phytoene
|2 NLM
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|a 87E4NJ6N51
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|a Geranylgeranyl-Diphosphate Geranylgeranyltransferase
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|a EC 2.5.1.32
|2 NLM
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|a Carotenoids
|2 NLM
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|a 36-88-4
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|a Chlorophyll
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|a 1406-65-1
|2 NLM
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|a HY5 protein, Arabidopsis
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Basic-Leucine Zipper Transcription Factors
|2 NLM
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|a PIF3 protein, Arabidopsis
|2 NLM
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|a Basic Helix-Loop-Helix Transcription Factors
|2 NLM
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|a Alagoz, Yagiz
|e verfasserin
|4 aut
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|a Welsch, Ralf
|e verfasserin
|4 aut
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|a Mortimer, Matthew D
|e verfasserin
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|a Pogson, Barry J
|e verfasserin
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|a Cazzonelli, Christopher I
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 4 vom: 12. Feb., Seite 1187-1204
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:75
|g year:2024
|g number:4
|g day:12
|g month:02
|g pages:1187-1204
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|u http://dx.doi.org/10.1093/jxb/erad443
|3 Volltext
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