|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM364394544 |
| 003 |
DE-627 |
| 005 |
20240126232012.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1002/adma.202306704
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1271.xml
|
| 035 |
|
|
|a (DE-627)NLM364394544
|
| 035 |
|
|
|a (NLM)37947789
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Park, Chan Ho
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Real-Time Spatiotemporal Measurement of Extracellular Signaling Molecules Using an Aptamer Switch-Conjugated Hydrogel Matrix
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.01.2024
|
| 500 |
|
|
|a Date Revised 26.01.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2023 Wiley-VCH GmbH.
|
| 520 |
|
|
|a Cells rely on secreted signaling molecules to coordinate essential biological functions including development, metabolism, and immunity. Unfortunately, such signaling processes remain difficult to measure with sufficient chemical specificity and temporal resolution. To address this need, an aptamer-conjugated hydrogel matrix that enables continuous fluorescent measurement of specific secreted analytes - in two dimensions, in real-time is developed. As a proof of concept, real-time imaging of inter-cellular cyclic adenosine 3',5'-monophosphate (cAMP) signals in Dictyostelium discoideum amoeba cells is performed. A set of aptamer switches that generate a rapid and reversible change in fluorescence in response to cAMP signals is engineered. By combining multiple switches with different dynamic ranges, measure cAMP concentrations spanning three orders of magnitude in a single experiment can be measured. These sensors are embedded within a biocompatible hydrogel on which cells are cultured and their cAMP secretions can be imaged using fluorescent microscopy. Using this aptamer-hydrogel material system, the first direct measurements of oscillatory cAMP signaling that correlate closely with previous indirect measurements are achieved. Using different aptamer switches, this approach can be generalized for measuring other secreted molecules to directly visualize diverse extracellular signaling processes and the biological effects that they trigger in recipient cells
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a aptamer
|
| 650 |
|
4 |
|a biosensor
|
| 650 |
|
4 |
|a cellular signaling
|
| 650 |
|
4 |
|a hydrogel
|
| 650 |
|
7 |
|a Cyclic AMP
|2 NLM
|
| 650 |
|
7 |
|a E0399OZS9N
|2 NLM
|
| 650 |
|
7 |
|a Hydrogels
|2 NLM
|
| 650 |
|
7 |
|a Adenosine
|2 NLM
|
| 650 |
|
7 |
|a K72T3FS567
|2 NLM
|
| 650 |
|
7 |
|a Oligonucleotides
|2 NLM
|
| 700 |
1 |
|
|a Thompson, Ian A P
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Newman, Sharon S
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hein, Linus A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lian, Xizhen
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fu, Kaiyu X
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pan, Jing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Eisenstein, Michael
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Soh, H Tom
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 4 vom: 08. Jan., Seite e2306704
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
|
| 773 |
1 |
8 |
|g volume:36
|g year:2024
|g number:4
|g day:08
|g month:01
|g pages:e2306704
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1002/adma.202306704
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 36
|j 2024
|e 4
|b 08
|c 01
|h e2306704
|