Rapid quantification of biological nitrogen fixation using optical spectroscopy

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 75(2024), 3 vom: 02. Feb., Seite 760-771
1. Verfasser:	Zhang, Haiyang (VerfasserIn)
Weitere Verfasser:	Plett, Jonathan M, Catunda, Karen L M, Churchill, Amber C, Moore, Ben D, Powell, Jeff R, Power, Sally A, Yang, Jinyan, Anderson, Ian C
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't isotopic composition nitrogen cycle spatial variation symbiotic fixation temporal variation δ15N Nitrogen Isotopes Nitrogen N762921K75

Beschreibung
Zusammenfassung:	© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Biological nitrogen fixation (BNF) provides a globally important input of nitrogen (N); its quantification is critical but technically challenging. Leaf reflectance spectroscopy offers a more rapid approach than traditional techniques to measure plant N concentration ([N]) and isotopes (δ15N). Here we present a novel method for rapidly and inexpensively quantifying BNF using optical spectroscopy. We measured plant [N], δ15N, and the amount of N derived from atmospheric fixation (Ndfa) following the standard traditional methodology using isotope ratio mass spectrometry (IRMS) from tissues grown under controlled conditions and taken from field experiments. Using the same tissues, we predicted the same three parameters using optical spectroscopy. By comparing the optical spectroscopy-derived results with traditional measurements (i.e. IRMS), the amount of Ndfa predicted by optical spectroscopy was highly comparable to IRMS-based quantification, with R2 being 0.90 (slope=0.90) and 0.94 (slope=1.02) (root mean square error for predicting legume δ15N was 0.38 and 0.43) for legumes grown in glasshouse and field, respectively. This novel application of optical spectroscopy facilitates BNF studies because it is rapid, scalable, low cost, and complementary to existing technologies. Moreover, the proposed method successfully captures the dynamic response of BNF to climate changes such as warming and drought
Beschreibung:	Date Completed 05.02.2024 Date Revised 26.07.2024 published: Print figshare: 10.6084/m9.figshare.20428344 Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erad426