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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c01835
|2 doi
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|a pubmed25n1211.xml
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|a (NLM)37877163
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|a DE-627
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|a eng
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|a Ibrahim, Mohd
|e verfasserin
|4 aut
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|a Adsorbing DNA to Mica by Cations
|b Influence of Valency and Ion Type
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 08.11.2023
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|a Date Revised 18.11.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Ion-mediated attraction between DNA and mica plays a crucial role in biotechnological applications and molecular imaging. Here, we combine molecular dynamics simulations and single-molecule atomic force microscopy experiments to characterize the detachment forces of single-stranded DNA at mica surfaces mediated by the metal cations Li+, Na+, K+, Cs+, Mg2+, and Ca2+. Ion-specific adsorption at the mica/water interface compensates (Li+ and Na+) or overcompensates (K+, Cs+, Mg2+, and Ca2+) the bare negative surface charge of mica. In addition, direct and water-mediated contacts are formed between the ions, the phosphate oxygens of DNA, and mica. The different contact types give rise to low- and high-force pathways and a broad distribution of detachment forces. Weakly hydrated ions, such as Cs+ and water-mediated contacts, lead to low detachment forces and high mobility of the DNA on the surface. Direct ion-DNA or ion-surface contacts lead to significantly higher forces. The comprehensive view gained from our combined approach allows us to highlight the most promising cations for imaging in physiological conditions: K+, which overcompensates the negative mica charge and induces long-ranged attractions. Mg2+ and Ca2+, which form a few specific and long-lived contacts to bind DNA with high affinity
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a mica
|2 NLM
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|a V8A1AW0880
|2 NLM
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|a Cations
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|a DNA
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|a 9007-49-2
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|a 059QF0KO0R
|2 NLM
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|a Wenzel, Christiane
|e verfasserin
|4 aut
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|a Lallemang, Max
|e verfasserin
|4 aut
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|a Balzer, Bizan N
|e verfasserin
|4 aut
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|a Schwierz, Nadine
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 39(2023), 44 vom: 07. Nov., Seite 15553-15562
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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|g volume:39
|g year:2023
|g number:44
|g day:07
|g month:11
|g pages:15553-15562
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|u http://dx.doi.org/10.1021/acs.langmuir.3c01835
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