LTP2 hypomorphs show genotype-by-environment interaction in early seedling traits in Arabidopsis thaliana
© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
| Veröffentlicht in: | The New phytologist. - 1979. - 241(2024), 1 vom: 16. Jan., Seite 253-266 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article LTP2 cuticle integrity gene-by-environment interaction hypocotyl hypomorph lipid transfer protein nongenetic variation phenotypic variation Arabidopsis Proteins |
| Zusammenfassung: | © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. Isogenic individuals can display seemingly stochastic phenotypic differences, limiting the accuracy of genotype-to-phenotype predictions. The extent of this phenotypic variation depends in part on genetic background, raising questions about the genes involved in controlling stochastic phenotypic variation. Focusing on early seedling traits in Arabidopsis thaliana, we found that hypomorphs of the cuticle-related gene LIPID TRANSFER PROTEIN 2 (LTP2) greatly increased variation in seedling phenotypes, including hypocotyl length, gravitropism and cuticle permeability. Many ltp2 hypocotyls were significantly shorter than wild-type hypocotyls while others resembled the wild-type. Differences in epidermal properties and gene expression between ltp2 seedlings with long and short hypocotyls suggest a loss of cuticle integrity as the primary determinant of the observed phenotypic variation. We identified environmental conditions that reveal or mask the increased variation in ltp2 hypomorphs and found that increased expression of its closest paralog LTP1 is necessary for ltp2 phenotypes. Our results illustrate how decreased expression of a single gene can generate starkly increased phenotypic variation in isogenic individuals in response to an environmental challenge |
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| Beschreibung: | Date Completed 18.12.2023 Date Revised 04.01.2025 published: Print-Electronic RefSeq: NP_181388.1, NP_181387.1, NP_568905.1, NP_568904.1, NP_190728.1, NP_187489.1, NP_973466.1, NP_179428.1, NP_179135.2, NP_195807.1, NP_680758.3, NP_190727.1, NP_001078707.1, NP_001078780.1, NP_192593.3 UpdateOf: bioRxiv. 2023 Sep 20:2023.05.11.540469. doi: 10.1101/2023.05.11.540469. - PMID 37214854 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.19334 |