HaMYBA-HabHLH1 regulatory complex and HaMYBF fine-tune red flower coloration in the corolla of sunflower (Helianthus annuus L.)

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 338(2024) vom: 01. Jan., Seite 111901
1. Verfasser:	Jiang, Wenhui (VerfasserIn)
Weitere Verfasser:	Jiang, Qinqin, Shui, Zhijie, An, Peipei, Shi, Shandang, Liu, Tianxiang, Zhang, Hanbing, Huang, Shuyi, Jing, Bing, Xiao, Enshi, Quan, Li, Liu, Jixia, Wang, Zhonghua
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Anthocyanin BHLH Flavonol MYB Sunflower Anthocyanins Flavonoids Plant Proteins

Beschreibung
Zusammenfassung:	Copyright © 2023 Elsevier B.V. All rights reserved. Sunflowers are well-known ornamental plants, while sunflowers with red corolla are rare and the mechanisms underlying red coloration remain unclear. Here, a comprehensive analysis of metabolomics and transcriptomics on flavonoid pathway was performed to investigate the molecular mechanisms underlying the differential color formation between red sunflower Pc103 and two yellow sunflowers (Yr17 and Y35). Targeted metabolomic analysis revealed higher anthocyanin levels but lower flavonol content in Pc103 compared to the yellow cultivars. RNA-sequencing and phylogenetic analysis identified multiple genes involved in the flavonoid pathway, including series of structural genes and three MYB and bHLH genes. Specifically, HaMYBA and HabHLH1 were up-regulated in Pc103, whereas HaMYBF exhibited reduced expression. HaMYBA was found to interact with HabHLH1 in vivo and in vitro, while HaMYBF does not. Transient expression analysis further revealed that HabHLH1 and HaMYBA cooperatively regulate increased expression of dihydroﬂavonol 4-reductase (DFR), leading to anthocyanin accumulation. On the other hand, ectopic expression of HaMYBF independently modulates flavonol synthase (FLS) expression, but hindered anthocyanin production. Collectively, our findings suggest that the up-regulation of HaMYBA and HabHLH1, as well as the down-regulation of HaMYBF, contribute to the red coloration in Pc103. It offers a theoretical basis for improving sunflower color through genetic engineering
Beschreibung:	Date Completed 16.12.2023 Date Revised 04.11.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2023.111901