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|a 10.1093/jxb/erad411
|2 doi
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|a pubmed24n1482.xml
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|a (DE-627)NLM363549307
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|a (NLM)37862584
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|a DE-627
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|e rakwb
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|a eng
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|a Ye, Shengjian
|e verfasserin
|4 aut
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|a Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 05.02.2024
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|a Date Revised 26.07.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kip-related proteins (KRPs). ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels. However, understanding of how ICK protein levels are regulated is very limited. We fused Arabidopsis ICK sequences to green fluorescent protein (GFP) and determined their effects on the fusion proteins in plants, yeast, and Escherichia coli. The N-terminal regions of ICKs drastically reduced GFP fusion protein levels in Arabidopsis plants. A number of short sequences of 10-20 residues were found to decrease GFP fusion protein levels when fused at the N-terminus or C-terminus. Three of the four short sequences from ICK3 showed a similar function in yeast. Intriguingly, three short sequences from ICK1 and ICK3 caused the degradation of the fusion proteins in E. coli. In addition, computational analyses showed that ICK proteins were mostly disordered and unstructured except for the conserved C-terminal region, suggesting that ICKs are intrinsically disordered proteins. This study has identified a number of short protein-destabilizing sequences, and evidence suggests that some of them may cause protein degradation through structural disorder and instability
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis
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|a CDK inhibitor
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|a ICK
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|a KRP
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|a intrinsically disordered region
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|a protein degradation
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|a short sequence
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|a ubiquitin-independent
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|a Arabidopsis Proteins
|2 NLM
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|a Cyclin-Dependent Kinase Inhibitor Proteins
|2 NLM
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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|a Cyclin-Dependent Kinases
|2 NLM
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|a EC 2.7.11.22
|2 NLM
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| 700 |
1 |
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|a Wang, Sheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chan, Ron
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Ling
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Hong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 3 vom: 02. Feb., Seite 772-788
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:75
|g year:2024
|g number:3
|g day:02
|g month:02
|g pages:772-788
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|u http://dx.doi.org/10.1093/jxb/erad411
|3 Volltext
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