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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-06-23-1081-SR
|2 doi
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|a pubmed24n1359.xml
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|a (NLM)37822097
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Fullem, Kiersten R
|e verfasserin
|4 aut
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| 245 |
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|a Unexpected Diversity of Pseudomonads Associated with Bacterial Leaf Spot of Cucurbits in the Southeastern United States
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| 264 |
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 01.04.2024
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|a Date Revised 01.04.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Bacterial leaf spot of cucurbits (BLS) is an emerging disease in the southeastern United States that is capable of causing widespread outbreaks under conducive conditions. Historically attributed solely to the bacterium Pseudomonas syringae pv. lachrymans, recent studies have identified additional P. syringae pathovars as causal agents of the disease. To further investigate the identity and diversity of P. syringae strains associated with BLS in the southeastern United States, 47 bacterial isolates were recovered from symptomatic cucurbits from Florida, Alabama, and Georgia. Strains were characterized using the LOPAT testing scheme, fluorescence, and pathogenicity to watermelon and squash seedlings. Thirty-eight fluorescent isolates underwent whole-genome sequencing and were further characterized with 16S rRNA, four gene multilocus sequence analysis (MLSA) phylogeny, and average nucleotide identity analysis. Thirty-four isolates were identified as members of the P. syringae species complex, including P. syringae sensu stricto (12), P. alliivorans (12), P. capsici (nine), and P. viridiflava (one). An additional four isolates were found to belong to the Pseudomonas genus outside of the syringae species complex, though they did not share 95% or greater average nucleotide identity to any validly published species and are believed to belong to three novel Pseudomonas species. These results reveal an unpredicted level of diversity of Pseudomonas strains associated with BLS in the region and show the benefits of whole-genome sequencing for strain identification. Identification of P. capsici, which is capable of causing disease at higher temperatures than P. syringae, as a causal agent of BLS may also affect management strategies in the future
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|a Journal Article
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|a pathogen diversity
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4 |
|a prokaryotes
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| 650 |
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4 |
|a vegetables
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| 650 |
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|a RNA, Ribosomal, 16S
|2 NLM
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| 650 |
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7 |
|a Nucleotides
|2 NLM
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| 700 |
1 |
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|a Pena, Michelle M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Potnis, Neha
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Goss, Erica M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Minsavage, Gerald V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Iriarte, Fanny B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Holland, Auston
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jones, Jeffrey B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Paret, Mathews L
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 108(2024), 3 vom: 01. März, Seite 592-598
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:108
|g year:2024
|g number:3
|g day:01
|g month:03
|g pages:592-598
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS-06-23-1081-SR
|3 Volltext
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