Plasma IgG aggregates as biomarkers for multiple sclerosis

Copyright © 2023. Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 256(2023) vom: 19. Nov., Seite 109801
1. Verfasser: Zhou, Wenbo (VerfasserIn)
Weitere Verfasser: Graner, Michael, Beseler, Cheryl, Domashevich, Timothy, Selva, Sean, Webster, Gill, Ledreux, Aurelie, Zizzo, Zoe, Lundt, Max, Alvarez, Enrique, Yu, Xiaoli
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antibody Biomarker Cytotoxicity Diagnosis IgG IgG aggregates IgG1 mehr... Immunoglobulin Multiple sclerosis Plasma Progressive MS SPMS Immunoglobulin G Biomarkers
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520 |a We recently reported that multiple sclerosis (MS) plasma contains IgG aggregates and induces complement-dependent neuronal cytotoxicity (Zhou et al., 2023). Using ELISA, we report herein that plasma IgG levels in the aggregates can be used as biomarkers for MS. We enriched the IgG aggregates from samples of two cohorts (190 MS and 160 controls) by collecting flow-through after plasma binding to Protein A followed by detection of IgG subclass. We show that there are significantly higher levels of IgG1, IgG3, and total IgG antibodies in MS IgG aggregates, with an AUC >90%; higher levels of IgG1 distinguish secondary progressive MS from relapsing-remitting MS (AUC = 91%). Significantly, we provided the biological rationale for MS plasma IgG biomarkers by demonstrating the strong correlation between IgG antibodies and IgG aggregate-induced neuronal cytotoxicity. These non-invasive, simple IgG-based blood ELISA assays can be adapted into clinical practice for diagnosing MS and SPMS and monitoring treatment responses 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Antibody 
650 4 |a Biomarker 
650 4 |a Cytotoxicity 
650 4 |a Diagnosis 
650 4 |a IgG 
650 4 |a IgG aggregates 
650 4 |a IgG1 
650 4 |a Immunoglobulin 
650 4 |a Multiple sclerosis 
650 4 |a Plasma 
650 4 |a Progressive MS 
650 4 |a SPMS 
650 7 |a Immunoglobulin G  |2 NLM 
650 7 |a Biomarkers  |2 NLM 
700 1 |a Graner, Michael  |e verfasserin  |4 aut 
700 1 |a Beseler, Cheryl  |e verfasserin  |4 aut 
700 1 |a Domashevich, Timothy  |e verfasserin  |4 aut 
700 1 |a Selva, Sean  |e verfasserin  |4 aut 
700 1 |a Webster, Gill  |e verfasserin  |4 aut 
700 1 |a Ledreux, Aurelie  |e verfasserin  |4 aut 
700 1 |a Zizzo, Zoe  |e verfasserin  |4 aut 
700 1 |a Lundt, Max  |e verfasserin  |4 aut 
700 1 |a Alvarez, Enrique  |e verfasserin  |4 aut 
700 1 |a Yu, Xiaoli  |e verfasserin  |4 aut 
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