Single-cell RNA sequencing of bronchoscopy specimens development of a rapid minimal handling protocol

Single-cell RNA sequencing of bronchoscopy specimens : development of a rapid minimal handling protocol

Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus presen...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 75(2023), 4 vom: 24. Okt., Seite 157-167
1. Verfasser: Gerayeli, Firoozeh V (VerfasserIn)
Weitere Verfasser: Milne, Stephen, Yang, Chen Xi, Li, Xuan, Guinto, Elizabeth, Yang, Julia Shun-Wei, Cheung, Chung Yan, Stach, Tara R, Shaipanich, Tawimas, Leung, Janice M, Sin, Don D
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't airway diseases bronchial brush bronchoalveolar lavage scRNA-seq single-cell RNA sequencing single-cell suspension
Beschreibung
Zusammenfassung:Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describe the development of a rapid and minimal handling protocol for preparing single-cell suspensions from bronchial brush specimens for scRNA-seq. Our optimized protocol maximizes cell recovery and cell quality and facilitates large-scale profiling of the airway transcriptome at single-cell resolution
Beschreibung:Date Completed 01.11.2023
Date Revised 17.11.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1940-9818
DOI:10.2144/btn-2023-0017