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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erad374
|2 doi
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|a pubmed24n1482.xml
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|a (DE-627)NLM362980748
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|a (NLM)37804484
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Guedes, Joana G
|e verfasserin
|4 aut
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| 245 |
1 |
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|a The leaf idioblastome of the medicinal plant Catharanthus roseus is associated with stress resistance and alkaloid metabolism
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 25.12.2023
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|a Date Revised 26.07.2024
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Catharanthus roseus leaves produce a range of monoterpenoid indole alkaloids (MIAs) that include low levels of the anticancer drugs vinblastine and vincristine. The MIA pathway displays a complex architecture spanning different subcellular and cell type localizations, and is under complex regulation. As a result, the development of strategies to increase the levels of the anticancer MIAs has remained elusive. The pathway involves mesophyll specialized idioblasts where the late unsolved biosynthetic steps are thought to occur. Here, protoplasts of C. roseus leaf idioblasts were isolated by fluorescence-activated cell sorting, and their differential alkaloid and transcriptomic profiles were characterized. This involved the assembly of an improved C. roseus transcriptome from short- and long-read data, IDIO+. It was observed that C. roseus mesophyll idioblasts possess a distinctive transcriptomic profile associated with protection against biotic and abiotic stresses, and indicative that this cell type is a carbon sink, in contrast to surrounding mesophyll cells. Moreover, it is shown that idioblasts are a hotspot of alkaloid accumulation, suggesting that their transcriptome may hold the key to the in-depth understanding of the MIA pathway and the success of strategies leading to higher levels of the anticancer drugs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Catharanthus roseus
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| 650 |
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|a Abiotic stress
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| 650 |
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|a anticancer drugs
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| 650 |
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|a biotic stress
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| 650 |
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4 |
|a fluorescence-activated cell sorting (FACS)
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| 650 |
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4 |
|a idioblasts
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| 650 |
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4 |
|a monoterpenoid indole alkaloids
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| 650 |
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4 |
|a protoplasts
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| 650 |
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4 |
|a transcriptome
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| 650 |
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4 |
|a vinblastine
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| 650 |
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|a Antineoplastic Agents
|2 NLM
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| 650 |
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|a Secologanin Tryptamine Alkaloids
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Ribeiro, Rogério
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carqueijeiro, Inês
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guimarães, Ana Luísa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bispo, Cláudia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Archer, John
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Azevedo, Herlander
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fonseca, Nuno A
|e verfasserin
|4 aut
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| 700 |
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|a Sottomayor, Mariana
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 1 vom: 01. Jan., Seite 274-299
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:75
|g year:2024
|g number:1
|g day:01
|g month:01
|g pages:274-299
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erad374
|3 Volltext
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|a AR
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|d 75
|j 2024
|e 1
|b 01
|c 01
|h 274-299
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