A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography

A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography

© James Birch et al. 2023.

Bibliographische Detailangaben
Veröffentlicht in:Journal of applied crystallography. - 1998. - 56(2023), Pt 5 vom: 01. Okt., Seite 1361-1370
1. Verfasser: Birch, James (VerfasserIn)
Weitere Verfasser: Kwan, Tristan O C, Judge, Peter J, Axford, Danny, Aller, Pierre, Butryn, Agata, Reis, Rosana I, Bada Juarez, Juan F, Vinals, Javier, Owen, Robin L, Nango, Eriko, Tanaka, Rie, Tono, Kensuke, Joti, Yasumasa, Tanaka, Tomoyuki, Owada, Shigeki, Sugahara, Michihiro, Iwata, So, Orville, Allen M, Watts, Anthony, Moraes, Isabel
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Journal of applied crystallography
Schlagworte:Journal Article A2A adenosine receptor archaerhodopsin-3 lipidic cubic phase membrane proteins serial crystallography structure-based drug design
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520 |a Serial crystallography has emerged as an important tool for structural studies of integral membrane proteins. The ability to collect data from micrometre-sized weakly diffracting crystals at room temperature with minimal radiation damage has opened many new opportunities in time-resolved studies and drug discovery. However, the production of integral membrane protein microcrystals in lipidic cubic phase at the desired crystal density and quantity is challenging. This paper introduces VIALS (versatile approach to high-density microcrystals in lipidic cubic phase for serial crystallography), a simple, fast and efficient method for preparing hundreds of microlitres of high-density microcrystals suitable for serial X-ray diffraction experiments at both synchrotron and free-electron laser sources. The method is also of great benefit for rational structure-based drug design as it facilitates in situ crystal soaking and rapid determination of many co-crystal structures. Using the VIALS approach, room-temperature structures are reported of (i) the archaerhodopsin-3 protein in its dark-adapted state and 110 ns photocycle intermediate, determined to 2.2 and 1.7 Å, respectively, and (ii) the human A2A adenosine receptor in complex with two different ligands determined to a resolution of 3.5 Å 
650 4 |a Journal Article 
650 4 |a A2A adenosine receptor 
650 4 |a archaerhodopsin-3 
650 4 |a lipidic cubic phase 
650 4 |a membrane proteins 
650 4 |a serial crystallography 
650 4 |a structure-based drug design 
700 1 |a Kwan, Tristan O C  |e verfasserin  |4 aut 
700 1 |a Judge, Peter J  |e verfasserin  |4 aut 
700 1 |a Axford, Danny  |e verfasserin  |4 aut 
700 1 |a Aller, Pierre  |e verfasserin  |4 aut 
700 1 |a Butryn, Agata  |e verfasserin  |4 aut 
700 1 |a Reis, Rosana I  |e verfasserin  |4 aut 
700 1 |a Bada Juarez, Juan F  |e verfasserin  |4 aut 
700 1 |a Vinals, Javier  |e verfasserin  |4 aut 
700 1 |a Owen, Robin L  |e verfasserin  |4 aut 
700 1 |a Nango, Eriko  |e verfasserin  |4 aut 
700 1 |a Tanaka, Rie  |e verfasserin  |4 aut 
700 1 |a Tono, Kensuke  |e verfasserin  |4 aut 
700 1 |a Joti, Yasumasa  |e verfasserin  |4 aut 
700 1 |a Tanaka, Tomoyuki  |e verfasserin  |4 aut 
700 1 |a Owada, Shigeki  |e verfasserin  |4 aut 
700 1 |a Sugahara, Michihiro  |e verfasserin  |4 aut 
700 1 |a Iwata, So  |e verfasserin  |4 aut 
700 1 |a Orville, Allen M  |e verfasserin  |4 aut 
700 1 |a Watts, Anthony  |e verfasserin  |4 aut 
700 1 |a Moraes, Isabel  |e verfasserin  |4 aut 
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