ER body-resident myrosinases and tryptophan specialized metabolism modulate root microbiota assembly

ER body-resident myrosinases and tryptophan specialized metabolism modulate root microbiota assembly

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 241(2023), 1 vom: 28. Jan., Seite 329-342
1. Verfasser: Basak, Arpan Kumar (VerfasserIn)
Weitere Verfasser: Piasecka, Anna, Hucklenbroich, Jana, Türksoy, Gözde Merve, Guan, Rui, Zhang, Pengfan, Getzke, Felix, Garrido-Oter, Ruben, Hacquard, Stephane, Strzałka, Kazimierz, Bednarek, Paweł, Yamada, Kenji, Nakano, Ryohei Thomas
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article endoplasmic reticulum (ER) body glucosinolate host-microbe interaction microbiota root exudate specialized metabolism thioglucosidase EC 3.2.1.147 Tryptophan mehr... 8DUH1N11BX Glycoside Hydrolases EC 3.2.1.- Soil
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520 |a Endoplasmic reticulum (ER) bodies are ER-derived structures that contain a large amount of PYK10 myrosinase, which hydrolyzes tryptophan (Trp)-derived indole glucosinolates (IGs). Given the well-described role of IGs in root-microbe interactions, we hypothesized that ER bodies in roots are important for interaction with soil-borne microbes at the root-soil interface. We used mutants impaired in ER bodies (nai1), ER body-resident myrosinases (pyk10bglu21), IG biosynthesis (myb34/51/122), and Trp specialized metabolism (cyp79b2b3) to profile their root microbiota community in natural soil, evaluate the impact of axenically collected root exudates on soil or synthetic microbial communities, and test their response to fungal endophytes in a mono-association setup. Tested mutants exhibited altered bacterial and fungal communities in rhizoplane and endosphere, respectively. Natural soils and bacterial synthetic communities treated with mutant root exudates exhibited distinctive microbial profiles from those treated with wild-type (WT) exudates. Most tested endophytes severely restricted the growth of cyp79b2b3, a part of which also impaired the growth of pyk10bglu21. Our results suggest that root ER bodies and their resident myrosinases modulate the profile of root-secreted metabolites and thereby influence root-microbiota interactions 
650 4 |a Journal Article 
650 4 |a endoplasmic reticulum (ER) body 
650 4 |a glucosinolate 
650 4 |a host-microbe interaction 
650 4 |a microbiota 
650 4 |a root exudate 
650 4 |a specialized metabolism 
650 7 |a thioglucosidase  |2 NLM 
650 7 |a EC 3.2.1.147  |2 NLM 
650 7 |a Tryptophan  |2 NLM 
650 7 |a 8DUH1N11BX  |2 NLM 
650 7 |a Glycoside Hydrolases  |2 NLM 
650 7 |a EC 3.2.1.-  |2 NLM 
650 7 |a Soil  |2 NLM 
700 1 |a Piasecka, Anna  |e verfasserin  |4 aut 
700 1 |a Hucklenbroich, Jana  |e verfasserin  |4 aut 
700 1 |a Türksoy, Gözde Merve  |e verfasserin  |4 aut 
700 1 |a Guan, Rui  |e verfasserin  |4 aut 
700 1 |a Zhang, Pengfan  |e verfasserin  |4 aut 
700 1 |a Getzke, Felix  |e verfasserin  |4 aut 
700 1 |a Garrido-Oter, Ruben  |e verfasserin  |4 aut 
700 1 |a Hacquard, Stephane  |e verfasserin  |4 aut 
700 1 |a Strzałka, Kazimierz  |e verfasserin  |4 aut 
700 1 |a Bednarek, Paweł  |e verfasserin  |4 aut 
700 1 |a Yamada, Kenji  |e verfasserin  |4 aut 
700 1 |a Nakano, Ryohei Thomas  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 241(2023), 1 vom: 28. Jan., Seite 329-342  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:241  |g year:2023  |g number:1  |g day:28  |g month:01  |g pages:329-342 
856 4 0 |u http://dx.doi.org/10.1111/nph.19289  |3 Volltext 
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