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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c01499
|2 doi
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|a pubmed24n1564.xml
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|a (DE-627)NLM362521409
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|a (NLM)37755825
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Hassler, Joseph F
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Discovery of Kinetic Trapping of Poloxamers inside Liposomes via Thermal Treatment
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 11.10.2023
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|a Date Revised 11.10.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Poloxamers, a class of biocompatible, commercially available amphiphilic block polymers (ABPs) comprising poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO) blocks, interact with phospholipid bilayers, resulting in altered mechanical and surface properties. These block copolymers are useful in a variety of applications including therapeutics for Duchenne muscular dystrophy, as cell membrane stabilizers, and for drug delivery, as liposome surface modifying agents. Hydrogen bonding between water and oxygen atoms in PEO and PPO units results in thermoresponsive behavior because the bound water shell around both blocks dehydrates as the temperature increases. This motivated an investigation of poloxamer-lipid bilayer interactions as a function of temperature and thermal history. In this study, we applied pulsed-field-gradient NMR spectroscopy to measure the fraction of chains bound to 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) liposomes between 10 and 50 °C. We measured an (11 ± 3)-fold increase in binding affinity at 37 °C relative to 27 °C. Moreover, following incubation at 37 °C, it takes weeks for the system to re-equilibrate at 25 °C. Such slow desorption kinetics suggests that at elevated temperatures polymer chains can pass through the bilayer and access the interior of the liposomes, a mechanism that is inaccessible at lower temperatures. We propose a molecular mechanism to explain this effect, which could have important ramifications on the cellular distribution of ABPs and could be exploited to modulate the mechanical and surface properties of liposomes and cell membranes
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Liposomes
|2 NLM
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|a Poloxamer
|2 NLM
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|a 106392-12-5
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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|a 3WJQ0SDW1A
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Phospholipids
|2 NLM
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| 650 |
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|a Water
|2 NLM
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| 650 |
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|a 059QF0KO0R
|2 NLM
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| 700 |
1 |
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|a Lawson, Megan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arroyo, Erika Cerna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bates, Frank S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hackel, Benjamin J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lodge, Timothy P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 39(2023), 40 vom: 10. Okt., Seite 14263-14274
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:39
|g year:2023
|g number:40
|g day:10
|g month:10
|g pages:14263-14274
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|u http://dx.doi.org/10.1021/acs.langmuir.3c01499
|3 Volltext
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