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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1002/aah.10188
|2 doi
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|a pubmed24n1554.xml
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|a (DE-627)NLM362461767
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|a (NLM)37749801
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Abraham, Taylor
|e verfasserin
|4 aut
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|a Detection and virulence of Lactococcus garvieae and L. petauri from four lakes in southern California
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 23.10.2023
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|a Date Revised 01.10.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © 2023 The Authors. Journal of Aquatic Animal Health published by Wiley Periodicals LLC on behalf of American Fisheries Society.
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|a OBJECTIVE: The first objective of the study aimed to detect the presence of Lactococcus petauri, L. garvieae, and L. formosensis in fish (n = 359) and environmental (n = 161) samples from four lakes near an affected fish farm in California during an outbreak in 2020. The second objective was to compare the virulence of the Lactococcus spp. in Rainbow Trout Oncorhynchus mykiss and Largemouth Bass Micropterus salmoides
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|a METHODS: Standard bacterial culture methods were used to isolate Lactococcus spp. from brain and posterior kidney of sampled fish from the four lakes. Quantitative PCR (qPCR) was utilized to detect Lactococcus spp. DNA in fish tissues and environmental samples from the four lakes. Laboratory controlled challenges were conducted by injecting fish intracoelomically with representative isolates of L. petauri (n = 17), L. garvieae (n = 2), or L. formosensis (n = 4), and monitored for 14 days postchallenge (dpc)
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|a RESULT: Lactococcus garvieae was isolated from the brains of two Largemouth Bass in one of the lakes. Lactococcus spp. were detected in 14 fish (8 Bluegills Lepomis macrochirus and 6 Largemouth Bass) from 3 out of the 4 lakes using a qPCR assay. Of the collected environmental samples, all 4 lakes tested positive for Lactococcus spp. in the soil samples, while 2 of the 4 lakes tested positive in the water samples through qPCR. Challenged Largemouth Bass did not show any signs of infection postinjection throughout the challenge period. Rainbow Trout infected with L. petauri showed clinical signs within 3 dpc and presented a significantly higher cumulative mortality (62.4%; p < 0.0001) at 14 dpc when compared to L. garvieae (0%) and L. formosensis (7.5%) treatments
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|a CONCLUSION: The study suggests that qPCR can be used for environmental DNA monitoring of Lactococcus spp. and demonstrates virulence diversity between the etiological agents of piscine lactococcosis
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Research Support, Non-U.S. Gov't
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|a emerging
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|a lactococcosis
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| 650 |
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|a salmonids
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|a Yazdi, Zeinab
|e verfasserin
|4 aut
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|a Littman, Eric
|e verfasserin
|4 aut
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|a Shahin, Khalid
|e verfasserin
|4 aut
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|a Heckman, Taylor I
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|4 aut
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|a Quijano Cardé, Eva Marie
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|4 aut
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|a Nguyen, Diem Thu
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|4 aut
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|a Hu, Ruixue
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|a Adkison, Mark
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|a Veek, Tresa
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|a Mukkatira, Kavery
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|a Richey, Christine
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|a Kwak, Kevin
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|a Mohammed, Haitham H
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|a Ortega, Cesar
|e verfasserin
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|a Avendaño-Herrera, Ruben
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|a Keleher, William
|e verfasserin
|4 aut
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|a LePage, Véronique
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|a Gardner, Ian
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|4 aut
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|a Welch, Timothy J
|e verfasserin
|4 aut
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|a Soto, Esteban
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 35(2023), 3 vom: 22. Sept., Seite 187-198
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnns
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| 773 |
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|g volume:35
|g year:2023
|g number:3
|g day:22
|g month:09
|g pages:187-198
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|u http://dx.doi.org/10.1002/aah.10188
|3 Volltext
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