GH3-mediated auxin inactivation attenuates multiple stages of lateral root development

© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 240(2023), 5 vom: 10. Dez., Seite 1900-1912
1. Verfasser: Wang, Qing (VerfasserIn)
Weitere Verfasser: De Gernier, Hugues, Duan, Xingliang, Xie, Yuanming, Geelen, Danny, Hayashi, Ken-Ishiro, Xuan, Wei, Geisler, Markus, Ten Tusscher, Kirsten, Beeckman, Tom, Vanneste, Steffen
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis thaliana DAO1 GH3 auxin auxin conjugation lateral root oscillation root Indoleacetic Acids Arabidopsis Proteins
Beschreibung
Zusammenfassung:© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
Lateral root (LR) positioning and development rely on the dynamic interplay between auxin production, transport but also inactivation. Nonetheless, how the latter affects LR organogenesis remains largely uninvestigated. Here, we systematically analyze the impact of the major auxin inactivation pathway defined by GRETCHEN HAGEN3-type (GH3) auxin conjugating enzymes and DIOXYGENASE FOR AUXIN OXIDATION1 (DAO1) in all stages of LR development using reporters, genetics and inhibitors in Arabidopsis thaliana. Our data demonstrate that the gh3.1/2/3/4/5/6 hextuple (gh3hex) mutants display a higher LR density due to increased LR initiation and faster LR developmental progression, acting epistatically over dao1-1. Grafting and local inhibitor applications reveal that root and shoot GH3 activities control LR formation. The faster LR development in gh3hex is associated with GH3 expression domains in and around developing LRs. The increase in LR initiation is associated with accelerated auxin response oscillations coinciding with increases in apical meristem size and LR cap cell death rates. Our research reveals how GH3-mediated auxin inactivation attenuates LR development. Local GH3 expression in LR primordia attenuates development and emergence, whereas GH3 effects on pre-initiation stages are indirect, by modulating meristem activities that in turn coordinate root growth with LR spacing
Beschreibung:Date Completed 03.11.2023
Date Revised 03.11.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19284