Upcycling of PET oligomers from chemical recycling processes to PHA by microbial co-cultivation
Copyright © 2023 Elsevier Ltd. All rights reserved.
Publié dans: | Waste management (New York, N.Y.). - 1999. - 172(2023) vom: 01. Dez., Seite 51-59 |
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Auteur principal: | |
Autres auteurs: | , , , , |
Format: | Article en ligne |
Langue: | English |
Publié: |
2023
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Accès à la collection: | Waste management (New York, N.Y.) |
Sujets: | Journal Article Co-cultivation Metabolic engineering PET oligomers Plastic upcycling Polyethylene terephthalate Polyhydroxyalkanoates Polyethylene Terephthalates terephthalic acid 6S7NKZ40BQ |
Résumé: | Copyright © 2023 Elsevier Ltd. All rights reserved. Polyethylene terephthalate (PET) is the most widely consumed polyester plastic and can be recycled by many chemical processes, of which glycolysis is most cost-effective and commercially viable. However, PET glycolysis produces oligomers due to incomplete depolymerization, which are undesirable by-products and require proper disposal. In this study, the PET oligomers from chemical recycling processes were completely bio-depolymerized into monomers and then used for the biosynthesis of biodegradable plastics polyhydroxyalkanoates (PHA) by co-cultivation of two engineered microorganisms Escherichia coli BL21 (DE3)-LCCICCG and Pseudomonas putida KT2440-ΔRDt-ΔZP46C-M. E. coli BL21 (DE3)-LCCICCG was used to secrete the PET hydrolase LCCICCG into the medium to directly depolymerize PET oligomers. P. putida KT2440-ΔRDt-ΔZP46C-M that mastered the metabolism of aromatic compounds was engineered to accelerate the hydrolysis of intermediate products mono-2-(hydroxyethyl) terephthalate (MHET) by expressing IsMHETase, and biosynthesize PHA using ultimate products terephthalate and ethylene glycol depolymerized from the PET oligomers. The population ratios of the two microorganisms during the co-cultivation were characterized by fluorescent reporter system, and revealed the collaboration of the two microorganisms to bio-depolymerize and bioconversion of PET oligomers in a single process. This study provides a biological strategy for the upcycling of PET oligomers and promotes the plastic circular economy |
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Description: | Date Completed 28.11.2023 Date Revised 28.11.2023 published: Print-Electronic Citation Status MEDLINE |
ISSN: | 1879-2456 |
DOI: | 10.1016/j.wasman.2023.08.048 |